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  Analysis of alanine aminotransferase in various organs of soybean (Glycine max) and in dependence of different nitrogen fertilisers during hypoxic stress

Rocha, M., Sodek, L., Licausi, F., Hameed, M. W., Dornelas, M. C., & van Dongen, J. T. (2010). Analysis of alanine aminotransferase in various organs of soybean (Glycine max) and in dependence of different nitrogen fertilisers during hypoxic stress. Amino Acids, 39(4), 1043-1053. doi:10.1007/s00726-010-0596-1.

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Rocha-2010-Analysis of alanine.pdf (beliebiger Volltext), 659KB
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Rocha, M.1, Autor           
Sodek, L.2, Autor
Licausi, F.1, Autor           
Hameed, M. W.2, Autor
Dornelas, M. C.2, Autor
van Dongen, J. T.1, Autor           
Affiliations:
1Energy Metabolism, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753325              
2External Organizations, ou_persistent22              

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Schlagwörter: glycine max soybean alanine aminotransferase hypoxic stress waterlogging nitrogen fertilisation anaerobic metabolism oxygen concentration medicago-truncatula arabidopsis roots seedlings glutamate respiration sequence wheat
 Zusammenfassung: Alanine aminotransferase (AlaAT) catalyses the reversible conversion of pyruvate and glutamate into alanine and oxoglutarate. In soybean, two subclasses were identified, each represented by two highly similar members. To investigate the role of AlaAT during hypoxic stress in soybean, changes in transcript level of both subclasses were analysed together with the enzyme activity and alanine content of the tissue. Moreover, the dependency of AlaAT activity and gene expression was investigated in relation to the source of nitrogen supplied to the plants. Using semi-quantitative PCR, GmAlaAT genes were determined to be highest expressed in roots and nodules. Under normal growth conditions, enzyme activity of AlaAT was detected in all organs tested, with lowest activity in the roots. Upon waterlogging-induced hypoxia, AlaAT activity increased strongly. Concomitantly, alanine accumulated. During re-oxygenation, AlaAT activity remained high, but the transcript level and the alanine content decreased. Our results show a role for AlaAT in the catabolism of alanine during the initial period of re-oxygenation following hypoxia. GmAlaAT also responded to nitrogen availability in the solution during waterlogging. Ammonium as nitrogen source induced both gene expression and enzyme activity of AlaAT more than when nitrate was supplied in the nutrient solution. The work presented here indicates that AlaAT might not only be important during hypoxia, but also during the recovery phase after waterlogging, when oxygen is available to the tissue again.

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Sprache(n): eng - English
 Datum: 2010-04-232010
 Publikationsstatus: Erschienen
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 Identifikatoren: ISI: ISI:000282177400011
DOI: 10.1007/s00726-010-0596-1
ISSN: 0939-4451
URI: ://000282177400011 http://www.springerlink.com/content/73141131215w1815/fulltext.pdf
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Titel: Amino Acids
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 39 (4) Artikelnummer: - Start- / Endseite: 1043 - 1053 Identifikator: -