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  Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX

Meinecke, L., Alawady, A., Schroda, M., Willows, R., Kobayashi, M. C., Niyogi, K. K., et al. (2010). Chlorophyll-deficient mutants of Chlamydomonas reinhardtii that accumulate magnesium protoporphyrin IX. Plant Molecular Biology, 72(6), 643-658. doi:10.1007/s11103-010-9604-9.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-23A2-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-23A3-7
Genre: Journal Article

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 Creators:
Meinecke, L.1, Author
Alawady, A.1, Author
Schroda, M.2, Author              
Willows, R.1, Author
Kobayashi, M. C.1, Author
Niyogi, K. K.1, Author
Grimm, B.1, Author
Beck, C. F.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753312              

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Free keywords: chlamydomonas mg protoporphyrin ix methyltransferase mutants mgprotoporphyrin ix accumulation gene expression signaling delta-aminolevulinic-acid cytochrome b(6)f complex light-harvesting complex adenosyl-l-methionine heat-shock genes photosystem-ii tetrapyrrole biosynthesis carotenoid biosynthesis singlet oxygen messenger-rna
 Abstract: Two Chlamydomonas reinhardtii mutants defective in CHLM encoding Mg-protoporphyrin IX methyltransferase (MgPMT) were identified. The mutants, one with a missense mutation (chlM-1) and a second mutant with a splicing defect (chlM-2), do not accumulate chlorophyll, are yellow in the dark and dim light, and their growth is inhibited at higher light intensities. They accumulate Mg-protoporphyrin IX (MgProto), the substrate of MgPMT and this may be the cause for their light sensitivity. In the dark, both mutants showed a drastic reduction in the amounts of core proteins of photosystems I and II and light-harvesting chlorophyll a/b-binding proteins. However, LHC mRNAs accumulated above wild-type levels. The accumulation of the transcripts of the LHC and other genes that were expressed at higher levels in the mutants during dark incubation was attenuated in the initial phase of light exposure. No regulatory effects of the constitutively 7- to 18-fold increased MgProto levels on gene expression were detected, supporting previous results in which MgProto and heme in Chlamydomonas were assigned roles as second messengers only in the transient activation of genes by light.

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Language(s): eng - English
 Dates: 2010
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000275556500007
DOI: 10.1007/s11103-010-9604-9
ISSN: 0167-4412
URI: ://000275556500007 http://www.springerlink.com/content/m5811802j8144514/fulltext.pdf
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Title: Plant Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 72 (6) Sequence Number: - Start / End Page: 643 - 658 Identifier: -