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  Characterization of the Branched-Chain Amino Acid Aminotransferase Enzyme Family in Tomato

Maloney, G. S., Kochevenko, A., Tieman, D. M., Tohge, T., Krieger, U., Zamir, D., et al. (2010). Characterization of the Branched-Chain Amino Acid Aminotransferase Enzyme Family in Tomato. Plant Physiology, 153(3), 925-936. doi:10.1104/pp.110.154922.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-23AE-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-842B-1
Genre: Journal Article

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 Creators:
Maloney, G. S.1, Author
Kochevenko, A.2, Author              
Tieman, D. M.1, Author
Tohge, T.2, Author              
Krieger, U.1, Author
Zamir, D.1, Author
Taylor, M. G.1, Author
Fernie, A. R.2, Author              
Klee, H. J.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: escherichia-coli arabidopsis-thaliana saccharomyces-cerevisiae spinach-chloroplasts leucine biosynthesis fatty-acids plants metabolism catabolism expression
 Abstract: Branched-chain amino acids (BCAAs) are synthesized in plants from branched-chain keto acids, but their metabolism is not completely understood. The interface of BCAA metabolism lies with branched-chain aminotransferases (BCAT) that catalyze both the last anabolic step and the first catabolic step. In this study, six BCAT genes from the cultivated tomato (Solanum lycopersicum) were identified and characterized. SlBCAT1, -2, -3, and -4 are expressed in multiple plant tissues, while SlBCAT5 and -6 were undetectable. SlBCAT1 and -2 are located in the mitochondria, SlBCAT3 and -4 are located in chloroplasts, while SlBCAT5 and -6 are located in the cytosol and vacuole, respectively. SlBCAT1, -2, -3, and -4 were able to restore growth of Escherichia coli BCAA auxotrophic cells, but SlBCAT1 and -2 were less effective than SlBCAT3 and -4 in growth restoration. All enzymes were active in the forward (BCAA synthesis) and reverse (branched-chain keto acid synthesis) reactions. SlBCAT3 and -4 exhibited a preference for the forward reaction, while SlBCAT1 and -2 were more active in the reverse reaction. While overexpression of SlBCAT1 or -3 in tomato fruit did not significantly alter amino acid levels, an expression quantitative trait locus on chromosome 3, associated with substantially higher expression of Solanum pennellii BCAT4, did significantly increase BCAA levels. Conversely, antisense-mediated reduction of SlBCAT1 resulted in higher levels of BCAAs. Together, these results support a model in which the mitochondrial SlBCAT1 and -2 function in BCAA catabolism while the chloroplastic SlBCAT3 and -4 function in BCAA synthesis.

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Language(s): eng - English
 Dates: 2010-05-042010
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000279400200004
DOI: 10.1104/pp.110.154922
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000279400200004http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2899903/pdf/925.pdf?tool=pmcentrez
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 153 (3) Sequence Number: - Start / End Page: 925 - 936 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438