English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Virus-Induced Gene Silencing of Plastidial Soluble Inorganic Pyrophosphatase Impairs Essential Leaf Anabolic Pathways and Reduces Drought Stress Tolerance in Nicotiana benthamiana

George, G. M., van der Merwe, M. J., Nunes-Nesi, A., Bauer, R., Fernie, A. R., Kossmann, J., et al. (2010). Virus-Induced Gene Silencing of Plastidial Soluble Inorganic Pyrophosphatase Impairs Essential Leaf Anabolic Pathways and Reduces Drought Stress Tolerance in Nicotiana benthamiana. Plant Physiology, 154(1), 55-66. doi:10.1104/pp.110.157776.

Item is

Files

show Files
hide Files
:
George-2010-Virus-Induced Gene S.pdf (Any fulltext), 844KB
Name:
George-2010-Virus-Induced Gene S.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
George, G. M.1, Author
van der Merwe, M. J.2, Author              
Nunes-Nesi, A.2, Author              
Bauer, R.1, Author
Fernie, A. R.2, Author              
Kossmann, J.3, Author              
Lloyd, J. R.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Carbohydrate Molecular Physiology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753344              

Content

show
hide
Free keywords: escherichia-coli pyrophosphatase strongly decreased expression adp-glucose pyrophosphorylase potato solanum-tuberosum starch synthesis arabidopsis-thaliana adpglucose pyrophosphorylase trehalose 6-phosphate gas-chromatography redox activation
 Abstract: The role of pyrophosphate in primary metabolism is poorly understood. Here, we report on the transient down-regulation of plastid-targeted soluble inorganic pyrophosphatase in Nicotiana benthamiana source leaves. Physiological and metabolic perturbations were particularly evident in chloroplastic central metabolism, which is reliant on fast and efficient pyrophosphate dissipation. Plants lacking plastidial soluble inorganic pyrophosphatase (psPPase) were characterized by increased pyrophosphate levels, decreased starch content, and alterations in chlorophyll and carotenoid biosynthesis, while constituents like amino acids (except for histidine, serine, and tryptophan) and soluble sugars and organic acids (except for malate and citrate) remained invariable from the control. Furthermore, translation of Rubisco was significantly affected, as observed for the amounts of the respective subunits as well as total soluble protein content. These changes were concurrent with the fact that plants with reduced psPPase were unable to assimilate carbon to the same extent as the controls. Furthermore, plants with lowered psPPase exposed to mild drought stress showed a moderate wilting phenotype and reduced vitality, which could be correlated to reduced abscisic acid levels limiting stomatal closure. Taken together, the results suggest that plastidial pyrophosphate dissipation through psPPase is indispensable for vital plant processes.

Details

show
hide
Language(s): eng - English
 Dates: 2010-07-082010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000281570000005
DOI: 10.1104/pp.110.157776
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000281570000005http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938153/pdf/55.pdf?tool=pmcentrez
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 154 (1) Sequence Number: - Start / End Page: 55 - 66 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438