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  Phloem small RNAs, nutrient stress responses, and systemic mobility

Buhtz, A., Pieritz, J., Springer, F., & Kehr, J. (2010). Phloem small RNAs, nutrient stress responses, and systemic mobility. BMC Plant Biology, 10, 64. doi:10.1186/1471-2229-10-64.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2441-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2442-C
Genre: Journal Article

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Buhtz, A.1, Author              
Pieritz, J.2, Author
Springer, F.1, Author              
Kehr, J.1, Author              
Affiliations:
1Micro- and Protein-Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753346              
2External Organizations, ou_persistent22              

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Free keywords: arabidopsis-thaliana phosphate homeostasis microrna expression copper homeostasis iron homeostasis plant micrornas brassica-napus mirna root identification
 Abstract: Background: Nutrient availabilities and needs have to be tightly coordinated between organs to ensure a balance between uptake and consumption for metabolism, growth, and defense reactions. Since plants often have to grow in environments with sub-optimal nutrient availability, a fine tuning is vital. To achieve this, information has to flow cell-to-cell and over long-distance via xylem and phloem. Recently, specific miRNAs emerged as a new type of regulating molecules during stress and nutrient deficiency responses, and miR399 was suggested to be a phloem-mobile long-distance signal involved in the phosphate starvation response. Results: We used miRNA microarrays containing all known plant miRNAs and a set of unknown small (s) RNAs earlier cloned from Brassica phloem sap [1], to comprehensively analyze the phloem response to nutrient deficiency by removing sulfate, copper or iron, respectively, from the growth medium. We show that phloem sap contains a specific set of sRNAs that is distinct from leaves and roots, and that the phloem also responds specifically to stress. Upon S and Cu deficiencies phloem sap reacts with an increase of the same miRNAs that were earlier characterized in other tissues, while no clear positive response to -Fe was observed. However, -Fe led to a reduction of Cu- and P-responsive miRNAs. We further demonstrate that under nutrient starvation miR399 and miR395 can be translocated through graft unions from wild type scions to rootstocks of the miRNA processing hen1-1 mutant. In contrast, miR171 was not transported. Translocation of miR395 led to a down-regulation of one of its targets in rootstocks, suggesting that this transport is of functional relevance, and that miR395, in addition to the well characterized miR399, could potentially act as a long-distance information transmitter. Conclusions: Phloem sap contains a specific set of sRNAs, of which some specifically accumulate in response to nutrient deprivation. From the observation that miR395 and miR399 are phloem-mobile in grafting experiments we conclude that translocatable miRNAs might be candidates for information-transmitting molecules, but that grafting experiments alone are not sufficient to convincingly assign a signaling function.

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Language(s): eng - English
 Dates: 2010-04-132010
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000277291700001
DOI: 10.1186/1471-2229-10-64
ISSN: 1471-2229 (Electronic) 1471-2229 (Linking)
URI: ://000277291700001 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923538/pdf/1471-2229-10-64.pdf?tool=pmcentrez
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Title: BMC Plant Biology
Source Genre: Journal
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Pages: - Volume / Issue: 10 Sequence Number: - Start / End Page: 64 Identifier: -