Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Identification of the 2-Hydroxyglutarate and Isovaleryl-CoA Dehydrogenases as Alternative Electron Donors Linking Lysine Catabolism to the Electron Transport Chain of Arabidopsis Mitochondria

Araujo, W. L., Ishizaki, K., Nunes-Nesi, A., Larson, T. R., Tohge, T., Krahnert, I., et al. (2010). Identification of the 2-Hydroxyglutarate and Isovaleryl-CoA Dehydrogenases as Alternative Electron Donors Linking Lysine Catabolism to the Electron Transport Chain of Arabidopsis Mitochondria. The Plant Cell, 22(5), 1549-1563. doi:10.1105/tpc.110.075630.

Item is

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Araujo-2010-Identification of th.pdf (beliebiger Volltext), 2MB
Name:
Araujo-2010-Identification of th.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Araujo, W. L.1, Autor           
Ishizaki, K.2, Autor
Nunes-Nesi, A.1, Autor           
Larson, T. R.2, Autor
Tohge, T.1, Autor           
Krahnert, I.1, Autor           
Witt, S.1, Autor           
Obata, T.1, Autor           
Schauer, N.1, Autor           
Graham, I. A.2, Autor
Leaver, C. J.2, Autor
Fernie, A. R.1, Autor           
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: flavoprotein-ubiquinone oxidoreductase leaf senescence beta-oxidation chlorophyll breakdown gas-chromatography extended darkness pipecolic acid amino-acids plants metabolism
 Zusammenfassung: The process of dark-induced senescence in plants is relatively poorly understood, but a functional electron-transfer flavoprotein/electron-transfer flavoprotein: ubiquinone oxidoreductase (ETF/ETFQO) complex, which supports respiration during carbon starvation, has recently been identified. Here, we studied the responses of Arabidopsis thaliana mutants deficient in the expression of isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase to extended darkness and other environmental stresses. Evaluations of the mutant phenotypes following carbon starvation induced by extended darkness identify similarities to those exhibited by mutants of the ETF/ETFQO complex. Metabolic profiling and isotope tracer experimentation revealed that isovaleryl-CoA dehydrogenase is involved in degradation of the branched-chain amino acids, phytol, and Lys, while 2-hydroxyglutarate dehydrogenase is involved exclusively in Lys degradation. These results suggest that isovaleryl-CoA dehydrogenase is the more critical for alternative respiration and that a series of enzymes, including 2-hydroxyglutarate dehydrogenase, plays a role in Lys degradation. Both physiological and metabolic phenotypes of the isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase mutants were not as severe as those observed for mutants of the ETF/ETFQO complex, indicating some functional redundancy of the enzymes within the process. Our results aid in the elucidation of the pathway of plant Lys catabolism and demonstrate that both isovaleryl-CoA dehydrogenase and 2-hydroxyglutarate dehydrogenase act as electron donors to the ubiquinol pool via an ETF/ETFQO-mediated route.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2010-05-272010
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: ISI:000279253200012
DOI: 10.1105/tpc.110.075630
ISSN: 1532-298X (Electronic)1040-4651 (Linking)
URI: ://000279253200012http://www.plantcell.org/content/22/5/1549.full.pdf
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: The Plant Cell
  Kurztitel : Plant C
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Rockville : American Society of Plant Physiologists
Seiten: - Band / Heft: 22 (5) Artikelnummer: - Start- / Endseite: 1549 - 1563 Identifikator: Anderer: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X