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  Y3IP1, a Nucleus-Encoded Thylakoid Protein, Cooperates with the Plastid-Encoded Ycf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis

Albus, C. A., Ruf, S., Schöttler, M. A., Lein, W., Kehr, J., & Bock, R. (2010). Y3IP1, a Nucleus-Encoded Thylakoid Protein, Cooperates with the Plastid-Encoded Ycf3 Protein in Photosystem I Assembly of Tobacco and Arabidopsis. The Plant Cell, 22(8), 2838-2855. doi:10.1105/tpc.110.073908.

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 Creators:
Albus, C. A.1, Author              
Ruf, S.1, Author              
Schöttler, M. A.2, Author              
Lein, W.3, Author              
Kehr, J.4, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
2Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753323              
3Gene Function, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753333              
4Micro- and Protein-Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753346              

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Free keywords: synechococcus sp pcc-7002 chlamydomonas-reinhardtii angstrom resolution membrane-proteins reverse genetics chlorophyll-a complex transformation efficient accumulation
 Abstract: The intricate assembly of photosystem I (PSI), a large multiprotein complex in the thylakoid membrane, depends on auxiliary protein factors. One of the essential assembly factors for PSI is encoded by ycf3 (hypothetical chloroplast reading frame number 3) in the chloroplast genome of algae and higher plants. To identify novel factors involved in PSI assembly, we constructed an epitope-tagged version of ycf3 from tobacco (Nicotiana tabacum) and introduced it into the tobacco chloroplast genome by genetic transformation. Immunoaffinity purification of Ycf3 complexes from the transplastomic plants identified a novel nucleus-encoded thylakoid protein, Y3IP1 (for Ycf3-interacting protein 1), that specifically interacts with the Ycf3 protein. Subsequent reverse genetics analysis of Y3IP1 function in tobacco and Arabidopsis thaliana revealed that knockdown of Y3IP1 leads to a specific deficiency in PSI but does not result in loss of Ycf3. Our data indicate that Y3IP1 represents a novel factor for PSI biogenesis that cooperates with the plastid genome-encoded Ycf3 in the assembly of stable PSI units in the thylakoid membrane.

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Language(s): eng - English
 Dates: 2010-09-032010
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000282270600024
DOI: 10.1105/tpc.110.073908
ISSN: 1532-298X (Electronic)1040-4651 (Linking)
URI: ://000282270600024http://www.plantcell.org/content/22/8/2838.full.pdf
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 22 (8) Sequence Number: - Start / End Page: 2838 - 2855 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X