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  A functional analysis of the pyrimidine catabolic pathway in Arabidopsis

Zrenner, R., Riegler, H., Marquard, C. R., Lange, P. R., Geserick, C., Bartosz, C. E., et al. (2009). A functional analysis of the pyrimidine catabolic pathway in Arabidopsis. New Phytologist, 183(1), 117-132. doi:10.1111/j.1469-8137.2009.02843.x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2491-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2492-5
Genre: Journal Article

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 Creators:
Zrenner, R.1, Author              
Riegler, H.1, 2, Author              
Marquard, C. R.3, Author
Lange, P. R.3, Author
Geserick, C.1, Author              
Bartosz, C. E.3, Author
Chen, C. T.3, Author
Slocum, R. D.3, Author
Affiliations:
1Nucleotides and Sugars, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753335              
2Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              
3External Organizations, ou_persistent22              

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Free keywords: arabidopsis catabolic pathway mutant nitrogen metabolism pyrimidine nucleotide cell-suspension cultures beta-alanine dihydropyrimidine dehydrogenase euglena-gracilis higher-plants cdna cloning metabolism thaliana degradation enzymes
 Abstract: Reductive catabolism of pyrimidine nucleotides occurs via a three-step pathway in which uracil is degraded to beta-alanine, CO2 and NH3 through sequential activities of dihydropyrimidine dehydrogenase (EC 1.3.1.2, PYD1), dihydropyrimidinase (EC 3.5.2.2, PYD2) and beta-ureidopropionase (EC 3.5.1.6, PYD3). A proposed function of this pathway, in addition to the maintenance of pyrimidine homeostasis, is the recycling of pyrimidine nitrogen to general nitrogen metabolism. PYD expression and catabolism of [2-C-14]-uracil are markedly elevated in response to nitrogen limitation in plants, which can utilize uracil as a nitrogen source. PYD1, PYD2 and PYD3 knockout mutants were used for functional analysis of this pathway in Arabidopsis. pyd mutants exhibited no obvious phenotype under optimal growing conditions. pyd2 and pyd3 mutants were unable to catabolize [2-C-14]-uracil or to grow on uracil as the sole nitrogen source. By contrast, catabolism of uracil was reduced by only 40% in pyd1 mutants, and pyd1 seedlings grew nearly as well as wild-type seedlings with a uracil nitrogen source. These results confirm PYD1 function and suggest the possible existence of another, as yet unknown, activity for uracil degradation to dihydrouracil in this plant. The localization of PYD-green fluorescent protein fusions in the plastid (PYD1), secretory system (PYD2) and cytosol (PYD3) suggests potentially complex metabolic regulation. New Phytologist (2009) 183: 117-132doi: 10.1111/j.1469-8137.2009.02843.x.

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Language(s): eng - English
 Dates: 2009-05-062009
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: ISI: ISI:000266610100013
DOI: 10.1111/j.1469-8137.2009.02843.x
ISSN: 1469-8137 (Electronic) 0028-646X (Linking)
URI: ://000266610100013 http://onlinelibrary.wiley.com/store/10.1111/j.1469-8137.2009.02843.x/asset/j.1469-8137.2009.02843.x.pdf?v=1&t=h0caxc6l&s=8024948097cacdd8072ee531f26998890d7c51d0
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Title: New Phytologist
Source Genre: Journal
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Pages: - Volume / Issue: 183 (1) Sequence Number: - Start / End Page: 117 - 132 Identifier: -