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  Temporally resolved GC-MS-based metabolic profiling of herbicide treated plants treated reveals that changes in polar primary metabolites alone can distinguish herbicides of differing mode of action

Trenkamp, S., Eckes, P., Busch, M., & Fernie, A. R. (2009). Temporally resolved GC-MS-based metabolic profiling of herbicide treated plants treated reveals that changes in polar primary metabolites alone can distinguish herbicides of differing mode of action. Metabolomics, 5(3), 277-291. doi:10.1007/s11306-008-0149-8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-24C9-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-84DA-8
Genre: Journal Article

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 Creators:
Trenkamp, S.1, Author              
Eckes, P.2, Author
Busch, M.2, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              

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Free keywords: herbicide mode of action gc-ms metabolic profiling arabidopsis-thaliana glyphosate accumulation assimilation metabonomics inhibition systems
 Abstract: We conducted a comprehensive metabolic phenotyping of primary metabolism of photosynthetic tissue of Arabidopsis thaliana following spray treatment with a number of commercially used herbicides using a well established gas-chromatography mass-spectrometry profiling method. Applying this technique we were able to identify and quantify in excess of 80 polar metabolites and based on a combination of co-elution with standards and prediction from the mass spectra a similar number of lipophillic components within two chromatographic runs. The herbicides selected were glufosinate, sulcotrione, AE944 [N2-(1-ethyl-3-phenylpropyl)-6-(1-fluoro-1-methylethyl)-1,3,5-triazine-2,4-diamine], foramsulfuron, benfuresate and glyphosate. We determined causal changes in the metabolite profiles by following their time-dependent changes using a serial sampling strategy. The resultant profiles were compared both by looking at the largest changes in a metabolite by metabolite manner and by performance of statistical analyses. These data revealed that analysis of the polar metabolites allows clear separation of the compounds under test. This finding is discussed in the context of current strategies for agrochemical discovery.

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Language(s): eng - English
 Dates: 2009-09-012009
 Publication Status: Published in print
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 Table of Contents: -
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 Identifiers: ISI: ISI:000269312400001
DOI: 10.1007/s11306-008-0149-8
ISSN: 1573-3890 (Electronic)
URI: ://000269312400001http://www.springerlink.com/content/c4517604mr34420m/fulltext.pdf
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Title: Metabolomics
Source Genre: Journal
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Publ. Info: New York : Springer
Pages: - Volume / Issue: 5 (3) Sequence Number: - Start / End Page: 277 - 291 Identifier: Other: 1573-3882
CoNE: https://pure.mpg.de/cone/journals/resource/1573-3882