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  The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

Schroeder, F., Lisso, J., Lange, P., & Muessig, C. (2009). The extracellular EXO protein mediates cell expansion in Arabidopsis leaves. BMC Plant Biology, 9, 20. doi:10.1186/1471-2229-9-20.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-24E5-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-24E6-9
Genre: Journal Article

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 Creators:
Schroeder, F.1, Author              
Lisso, J.1, Author              
Lange, P.2, Author
Muessig, C.1, 3, Author              
Affiliations:
1Brassinosteroids, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753314              
2External Organizations, ou_persistent22              
3Developmental Physiology and Genomics, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753313              

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Free keywords: plant transformation gene-expression wall proteins thaliana brassinosteroids proliferation proteomics exordium vectors kinase
 Abstract: Background: The EXO (EXORDIUM) gene was identified as a potential mediator of brassinosteroid (BR)-promoted growth. It is part of a gene family with eight members in Arabidopsis. EXO gene expression is under control of BR, and EXO overexpression promotes shoot and root growth. In this study, the consequences of loss of EXO function are described. Results: The exo loss of function mutant showed diminished leaf and root growth and reduced biomass production. Light and scanning electron microscopy analyses revealed that impaired leaf growth is due to reduced cell expansion. Epidermis, palisade, and spongy parenchyma cells were smaller in comparison to the wild-type. The exo mutant showed reduced brassinolide-induced cotyledon and hypocotyl growth. In contrast, exo roots were significantly more sensitive to the inhibitory effect of synthetic brassinolide. Apart from reduced growth, exo did not show severe morphological abnormalities. Gene expression analyses of leaf material identified genes that showed robust EXO-dependent expression. Growth-related genes such as WAK1, EXP5, and KCS1, and genes involved in primary and secondary metabolism showed weaker expression in exo than in wild-type plants. However, the vast majority of BR-regulated genes were normally expressed in exo. HA-and GFP-tagged EXO proteins were targeted to the apoplast. Conclusion: The EXO gene is essential for cell expansion in leaves. Gene expression patterns and growth assays suggest that EXO mediates BR-induced leaf growth. However, EXO does not control BR-levels or BR-sensitivity in the shoot. EXO presumably is involved in a signalling process which coordinates BR-responses with environmental or developmental signals. The hypersensitivity of exo roots to BR suggests that EXO plays a diverse role in the control of BR responses in the root.

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Language(s): eng - English
 Dates: 2009-02-132009
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000265062100001
DOI: 10.1186/1471-2229-9-20
ISSN: 1471-2229 (Electronic) 1471-2229 (Linking)
URI: ://000265062100001 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2661892/pdf/1471-2229-9-20.pdf?tool=pmcentrez
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Title: BMC Plant Biology
Source Genre: Journal
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Pages: - Volume / Issue: 9 Sequence Number: - Start / End Page: 20 Identifier: -