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  Identification of Nutrient-Responsive Arabidopsis and Rapeseed MicroRNAs by Comprehensive Real-Time Polymerase Chain Reaction Profiling and Small RNA Sequencing

Pant, B. D., Musialak-Lange, M., Nuc, P., May, P., Buhtz, A., Kehr, J., et al. (2009). Identification of Nutrient-Responsive Arabidopsis and Rapeseed MicroRNAs by Comprehensive Real-Time Polymerase Chain Reaction Profiling and Small RNA Sequencing. Plant Physiology, 150(3), 1541-1555. doi:10.1104/pp.109.139139.

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Pant-2009-Identification of Nu.pdf (beliebiger Volltext), 3MB
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Pant-2009-Identification of Nu.pdf
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Pant, B. D.1, Autor           
Musialak-Lange, M.1, Autor           
Nuc, P.1, Autor           
May, P.2, Autor           
Buhtz, A.3, Autor           
Kehr, J.3, Autor           
Walther, D.2, Autor           
Scheible, W.-R.1, Autor           
Affiliations:
1Molecular Genomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753328              
2BioinformaticsCIG, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753303              
3Micro- and Protein-Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753346              

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Schlagwörter: stress-regulated micrornas receptor-like kinase nitrogen limitation medicago-truncatula plant micrornas rt-pcr phosphate homeostasis systemic regulation target recognition protein-synthesis
 Zusammenfassung: Comprehensive expression profiles of Arabidopsis (Arabidopsis thaliana) MIRNA genes and mature microRNAs (miRs) are currently not available. We established a quantitative real-time polymerase chain reaction platform that allows rapid and sensitive quantification of 177 Arabidopsis primary miR transcripts (pri-miRs). The platform was used to detect phosphorus (P) or nitrogen (N) status-responsive pri-miR species. Several pri-miR169 species as well as pri-miR398a were found to be repressed during N limitation, whereas during P limitation, pri-miR778, pri-miR827, and pri-miR399 species were induced and pri-miR398a was repressed. The corresponding responses of the biologically active, mature miRs were confirmed using specific stem-loop reverse transcription primer quantitative polymerase chain reaction assays and small RNA sequencing. Interestingly, the latter approach also revealed high abundance of some miR star strands. Bioinformatic analysis of small RNA sequences with a modified miRDeep algorithm led to the identification of the novel P limitation-induced miR2111, which is encoded by two loci in the Arabidopsis genome. Furthermore, miR2111, miR169, a miR827-like sequence, and the abundances of several miR star strands were found to be strongly dependent on P or N status in rapeseed (Brassica napus) phloem sap, flagging them as candidate systemic signals. Taken together, these results reveal the existence of complex small RNA-based regulatory networks mediating plant adaptation to mineral nutrient availability.

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Sprache(n): eng - English
 Datum: 2009-05-262009
 Publikationsstatus: Erschienen
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 Identifikatoren: ISI: ISI:000268696800036
DOI: 10.1104/pp.109.139139
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000268696800036 http://www.jstor.org/stable/pdfplus/40538114.pdf
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Titel: Plant Physiology
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 150 (3) Artikelnummer: - Start- / Endseite: 1541 - 1555 Identifikator: -