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  A Redox-Mediated Modulation of Stem Bolting in Transgenic Nicotiana sylvestris Differentially Expressing the External Mitochondrial NADPH Dehydrogenase

Liu, Y. J., Nunes-Nesi, A., Wallstrom, S. V., Lager, I., Michalecka, A. M., Norberg, F. E. B., et al. (2009). A Redox-Mediated Modulation of Stem Bolting in Transgenic Nicotiana sylvestris Differentially Expressing the External Mitochondrial NADPH Dehydrogenase. Plant Physiology, 150(3), 1248-1259. doi:10.1104/pp.109.136242.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-255F-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-84B2-D
Genre: Journal Article

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 Creators:
Liu, Y. J.1, Author
Nunes-Nesi, A.2, Author              
Wallstrom, S. V.1, Author
Lager, I.1, Author
Michalecka, A. M.1, Author
Norberg, F. E. B.1, Author
Widell, S.1, Author
Fredlund, K. M.1, Author
Fernie, A. R.2, Author              
Rasmusson, A. G.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: alternative nad(p)h dehydrogenases higher-plant mitochondria flowering-time control mads-box genes arabidopsis-thaliana complex-i malate-dehydrogenase respiratory-chain tomato plants photosynthetic performance
 Abstract: Cytosolic NADPH can be directly oxidized by a calcium-dependent NADPH dehydrogenase, NDB1, present in the plant mitochondrial electron transport chain. However, little is known regarding the impact of modified cytosolic NADPH reduction levels on growth and metabolism. Nicotiana sylvestris plants overexpressing potato (Solanum tuberosum) NDB1 displayed early bolting, whereas sense suppression of the same gene led to delayed bolting, with consequential changes in flowering time. The phenotype was dependent on light irradiance but not linked to any change in biomass accumulation. Whereas the leaf NADPH/NADP(+) ratio was unaffected, the stem NADPH/NADP(+) ratio was altered following the genetic modification and strongly correlated with the bolting phenotype. Metabolic profiling of the stem showed that the NADP(H) change affected relatively few, albeit central, metabolites, including 2-oxoglutarate, glutamate, ascorbate, sugars, and hexose-phosphates. Consistent with the phenotype, the modified NDB1 level also affected the expression of putative floral meristem identity genes of the SQUAMOSA and LEAFY types. Further evidence for involvement of the NADPH redox in stem development was seen in the distinct decrease in the stem apex NADPH/NADP(+) ratio during bolting. Additionally, the potato NDB1 protein was specifically detected in mitochondria, and a survey of its abundance in major organs revealed that the highest levels are found in green stems. These results thus strongly suggest that NDB1 in the mitochondrial electron transport chain can, by modifying cell redox levels, specifically affect developmental processes.

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Language(s): eng - English
 Dates: 2009-05-122009
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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 Identifiers: ISI: ISI:000268696800012
DOI: 10.1104/pp.109.136242
ISSN: 0032-0889 (Print)0032-0889 (Linking)
URI: ://000268696800012http://www.jstor.org/stable/pdfplus/40538090.pdf
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 150 (3) Sequence Number: - Start / End Page: 1248 - 1259 Identifier: ISSN: 0032-0889
CoNE: /journals/resource/991042744294438