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  Global Changes in the Transcript and Metabolic Profiles during Symbiotic Nitrogen Fixation in Phosphorus-Stressed Common Bean Plants

Hernandez, G., Valdes-Lopez, O., Ramirez, M., Goffard, N., Weiller, G., Aparicio-Fabre, R., et al. (2009). Global Changes in the Transcript and Metabolic Profiles during Symbiotic Nitrogen Fixation in Phosphorus-Stressed Common Bean Plants. Plant Physiology, 151(3), 1221-1238. doi:10.1104/pp.109.143842.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-25B4-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-25B5-F
Genre: Journal Article

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 Creators:
Hernandez, G.1, Author
Valdes-Lopez, O.1, Author
Ramirez, M.1, Author
Goffard, N.1, Author
Weiller, G.1, Author
Aparicio-Fabre, R.1, Author
Fuentes, S. I.1, Author
Erban, A.2, Author              
Kopka, J.2, Author              
Udvardi, M. K.3, Author              
Vance, C. P.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              
3Molecular Plant Nutrition, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753321              

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Free keywords: gene-expression data phaseolus-vulgaris l. lotus-japonicus p-deficiency n-2 fixation membrane phospholipids phosphate deprivation arabidopsis-thaliana root development soybean nodules
 Abstract: Phosphorus (P) deficiency is widespread in regions where the common bean (Phaseolus vulgaris), the most important legume for human consumption, is produced, and it is perhaps the factor that most limits nitrogen fixation. Global gene expression and metabolome approaches were used to investigate the responses of nodules from common bean plants inoculated with Rhizobium tropici CIAT899 grown under P-deficient and P-sufficient conditions. P-deficient inoculated plants showed drastic reduction in nodulation and nitrogenase activity as determined by acetylene reduction assay. Nodule transcript profiling was performed through hybridization of nylon filter arrays spotted with cDNAs, approximately 4,000 unigene set, from the nodule and P-deficient root library. A total of 459 genes, representing different biological processes according to updated annotation using the UniProt Knowledgebase database, showed significant differential expression in response to P: 59% of these were induced in P-deficient nodules. The expression platform for transcription factor genes based in quantitative reverse transcriptase-polymerase chain reaction revealed that 37 transcription factor genes were differentially expressed in P-deficient nodules and only one gene was repressed. Data from nontargeted metabolic profiles indicated that amino acids and other nitrogen metabolites were decreased, while organic and polyhydroxy acids were accumulated, in P-deficient nodules. Bioinformatics analyses using MapMan and PathExpress software tools, customized to common bean, were utilized for the analysis of global changes in gene expression that affected overall metabolism. Glycolysis and glycerolipid metabolism, and starch and Suc metabolism, were identified among the pathways significantly induced or repressed in P-deficient nodules, respectively.

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Language(s): eng - English
 Dates: 2009-09-172009
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000271430500026
DOI: 10.1104/pp.109.143842
ISSN: 1532-2548 (Electronic) 0032-0889 (Linking)
URI: ://000271430500026 http://www.jstor.org/stable/pdfplus/40537949.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 151 (3) Sequence Number: - Start / End Page: 1221 - 1238 Identifier: -