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  An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians

Depuydt, S., Trenkamp, S., Fernie, A. R., Elftieh, S., Renou, J. P., Vuylsteke, M., et al. (2009). An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians. Plant Physiology, 149(3), 1366-1386. doi:10.1104/pp.108.131805.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-25F9-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8486-2
Genre: Journal Article

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 Creators:
Depuydt, S.1, Author
Trenkamp, S.2, Author              
Fernie, A. R.2, Author              
Elftieh, S.1, Author
Renou, J. P.1, Author
Vuylsteke, M.1, Author
Holsters, M.1, Author
Vereecke, D.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: plant-pathogen interactions leafy gall formation arabidopsis-thaliana carbohydrate-metabolism defense responses gene-expression powdery mildew plasmodiophora-brassicae differential regulation signal-transduction
 Abstract: Rhodococcus fascians is a Gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. Global transcriptomics using microarrays combined with profiling of primary metabolites on infected Arabidopsis (Arabidopsis thaliana) plants revealed that this actinomycete modulated pathways to convert its host into a niche. The transcript data demonstrated that R. fascians leaves a very characteristic mark on Arabidopsis with a pronounced cytokinin response illustrated by the activation of cytokinin perception, signal transduction, and homeostasis. The microarray data further suggested active suppression of an oxidative burst during the R. fascians pathology, and comparison with publicly available transcript data sets implied a central role for auxin in the prevention of plant defense activation. Gene Ontology categorization of the differentially expressed genes hinted at a significant impact of infection on the primary metabolism of the host, which was confirmed by subsequent metabolite profiling. The much higher levels of sugars and amino acids in infected plants are presumably accessed by the bacteria as carbon and nitrogen sources to support epiphytic and endophytic colonization. Hexoses, accumulating from a significantly increased invertase activity, possibly inhibited the expression of photosynthesis genes and photosynthetic activity in infected leaves. Altogether, these changes are indicative of sink development in symptomatic tissues. The metabolomics data furthermore point to the possible occurrence of secondary signaling during the interaction, which might contribute to symptom development. These data are placed in the context of regulation of bacterial virulence gene expression, suppression of defense, infection phenotype, and niche establishment.

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Language(s): eng - English
 Dates: 2009-01-022009
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: ISI:000263843700014
DOI: 10.1104/pp.108.131805
ISSN: 0032-0889 (Print)0032-0889 (Linking)
URI: ://000263843700014http://www.jstor.org/stable/pdfplus/40537722.pdf
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 149 (3) Sequence Number: - Start / End Page: 1366 - 1386 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438