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  Expression profiling of rice cultivars differing in their tolerance to long-term drought stress

Degenkolbe, T., Do, P. T., Zuther, E., Repsilber, D., Walther, D., Hincha, D. K., et al. (2009). Expression profiling of rice cultivars differing in their tolerance to long-term drought stress. Plant Molecular Biology, 69(1-2), 133-153. doi:10.1007/s11103-008-9412-7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-25FC-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-1086-A
Genre: Journal Article

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Degenkolbe-2009-Expression profiling.pdf (Any fulltext), 509KB
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 Creators:
Degenkolbe, T.1, Author              
Do, P. T.1, Author              
Zuther, E.1, Author              
Repsilber, D.2, Author              
Walther, D.3, Author              
Hincha, D. K.1, Author              
Koehl, K. I.4, Author              
Affiliations:
1Transcript Profiling, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753306              
2BioinformaticsCRG, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753315              
3BioinformaticsCIG, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753303              
4Plant Cultivation and Transformation, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753305              

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Free keywords: abiotic stress expression profiling gene x environment interaction qtl water use efficiency water potential leaf senescence crop plants arabidopsis-thaliana genotypic variation osmotic adjustment genetic-analysis cdna microarray resistance responses tool
 Abstract: Understanding the molecular basis of plant performance under water-limiting conditions will help to breed crop plants with a lower water demand. We investigated the physiological and gene expression response of drought-tolerant (IR57311 and LC-93-4) and drought-sensitive (Nipponbare and Taipei 309) rice (Oryza sativa L.) cultivars to 18 days of drought stress in climate chamber experiments. Drought stressed plants grew significantly slower than the controls. Gene expression profiles were measured in leaf samples with the 20 K NSF oligonucleotide microarray. A linear model was fitted to the data to identify genes that were significantly regulated under drought stress. In all drought stressed cultivars, 245 genes were significantly repressed and 413 genes induced. Genes differing in their expression pattern under drought stress between tolerant and sensitive cultivars were identified by the genotype x environment (G x E) interaction term. More genes were significantly drought regulated in the sensitive than in the tolerant cultivars. Localizing all expressed genes on the rice genome map, we checked which genes with a significant G x E interaction co-localized with published quantitative trait loci regions for drought tolerance. These genes are more likely to be important for drought tolerance in an agricultural environment. To identify the metabolic processes with a significant G x E effect, we adapted the analysis software MapMan for rice. We found a drought stress induced shift toward senescence related degradation processes that was more pronounced in the sensitive than in the tolerant cultivars. In spite of higher growth rates and water use, more photosynthesis related genes were down-regulated in the tolerant than in the sensitive cultivars.

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Language(s): eng - English
 Dates: 2008-10-222009
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: ISI:000261181700011
DOI: 10.1007/s11103-008-9412-7
ISSN: 0167-4412 (Print)0167-4412 (Linking)
URI: ://000261181700011http://www.springerlink.com/content/m032g8g6187hnk87/fulltext.pdf
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Title: Plant Molecular Biology
  Other : Plant Mol. Biol.
Source Genre: Journal
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Publ. Info: The Hague : Martinus Nijhoff/Dr. W. Junk
Pages: - Volume / Issue: 69 (1-2) Sequence Number: - Start / End Page: 133 - 153 Identifier: ISSN: 0167-4412
CoNE: /journals/resource/954925482643