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  Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in Arabidopsis thaliana seedlings

Bischoff, V., Cookson, S. J., Wu, S., & Scheible, W.-R. (2009). Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in Arabidopsis thaliana seedlings. Journal of Experimental Botany, 60(3), 955-965. doi:10.1093/jxb/ern344.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2625-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0017-DF21-D
Genre: Journal Article

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Bischoff-2009-Thaxtomin A affects.pdf (Any fulltext), 519KB
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Bischoff, V.1, Author              
Cookson, S. J.1, Author              
Wu, S.2, Author
Scheible, W.-R.1, Author              
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1Molecular Genomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753328              
2External Organizations, ou_persistent22              

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Free keywords: arabidopsis thaliana callose cellulose defence response isoxaben lignin microtubule phytotoxin streptomyces thaxtomin cellulose-synthesis inhibitor suspension-cultured cells disease resistance synthase activity biosynthesis growth isoxaben homogalacturonan identification elongation
 Abstract: Thaxtomin A, a phytotoxin produced by Streptomyces eubacteria, is suspected to act as a natural cellulose synthesis inhibitor. This view is confirmed by the results obtained from new chemical, molecular, and microscopic analyses of Arabidopsis thaliana seedlings treated with thaxtomin A. Cell wall analysis shows that thaxtomin A reduces crystalline cellulose, and increases pectins and hemicellulose in the cell wall. Treatment with thaxtomin A also changes the expression of genes involved in primary and secondary cellulose synthesis as well as genes associated with pectin metabolism and cell wall remodelling, in a manner nearly identical to isoxaben. In addition, it induces the expression of several defence-related genes and leads to callose deposition. Defects in cellulose synthesis cause ectopic lignification phenotypes in A. thaliana, and it is shown that lignification is also triggered by thaxtomin A, although in a pattern different from isoxaben. Spinning disc confocal microscopy further reveals that thaxtomin A depletes cellulose synthase complexes from the plasma membrane and results in the accumulation of these particles in a small microtubule-associated compartment. The results provide new and clear evidence for thaxtomin A having a strong impact on cellulose synthesis, thus suggesting that this is its primary mode of action.

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Language(s): eng - English
 Dates: 2009-03-102009
 Publication Status: Published in print
 Pages: -
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 Identifiers: ISI: ISI:000264189900022
DOI: 10.1093/jxb/ern344
ISSN: 1460-2431 (Electronic)0022-0957 (Linking)
URI: ://000264189900022http://jxb.oxfordjournals.org/content/60/3/955.full.pdf
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Title: Journal of Experimental Botany
Source Genre: Journal
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Pages: - Volume / Issue: 60 (3) Sequence Number: - Start / End Page: 955 - 965 Identifier: -