English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Deciphering Transcriptional and Metabolic Networks Associated with Lysine Metabolism during Arabidopsis Seed Development

Angelovici, R., Fait, A., Zhu, X. H., Szymanski, J., Feldmesser, E., Fernie, A. R., et al. (2009). Deciphering Transcriptional and Metabolic Networks Associated with Lysine Metabolism during Arabidopsis Seed Development. Plant Physiology, 151(4), 2058-2072. doi:10.1104/pp.109.145631.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-263D-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8476-6
Genre: Journal Article

Files

show Files
hide Files
:
Angelovici-2009-Deciphering Transcri.pdf (Any fulltext), 3MB
Name:
Angelovici-2009-Deciphering Transcri.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Angelovici, R.1, Author
Fait, A.2, Author              
Zhu, X. H.1, Author
Szymanski, J.3, Author              
Feldmesser, E.1, Author
Fernie, A. R.2, Author              
Galili, G.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              

Content

show
hide
Free keywords: gas-chromatography amino-acids threonine deaminase alternative oxidase gene-expression tomato plants catabolism system growth photosynthesis
 Abstract: In order to elucidate transcriptional and metabolic networks associated with lysine (Lys) metabolism, we utilized developing Arabidopsis (Arabidopsis thaliana) seeds as a system in which Lys synthesis could be stimulated developmentally without application of chemicals and coupled this to a T-DNA insertion knockout mutation impaired in Lys catabolism. This seed-specific metabolic perturbation stimulated Lys accumulation starting from the initiation of storage reserve accumulation. Our results revealed that the response of seed metabolism to the inducible alteration of Lys metabolism was relatively minor; however, that which was observable operated in a modular manner. They also demonstrated that Lys metabolism is strongly associated with the operation of the tricarboxylic acid cycle while largely disconnected from other metabolic networks. In contrast, the inducible alteration of Lys metabolism was strongly associated with gene networks, stimulating the expression of hundreds of genes controlling anabolic processes that are associated with plant performance and vigor while suppressing a small number of genes associated with plant stress interactions. The most pronounced effect of the developmentally inducible alteration of Lys metabolism was an induction of expression of a large set of genes encoding ribosomal proteins as well as genes encoding translation initiation and elongation factors, all of which are associated with protein synthesis. With respect to metabolic regulation, the inducible alteration of Lys metabolism was primarily associated with altered expression of genes belonging to networks of amino acids and sugar metabolism. The combined data are discussed within the context of network interactions both between and within metabolic and transcriptional control systems.

Details

show
hide
Language(s): eng - English
 Dates: 2009-09-292009
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: ISI:000272443500031
DOI: 10.1104/pp.109.145631
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000272443500031http://www.jstor.org/stable/pdfplus/40537640.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 151 (4) Sequence Number: - Start / End Page: 2058 - 2072 Identifier: ISSN: 0032-0889
CoNE: /journals/resource/991042744294438