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  Insensitivity of chloroplast gene expression to DNA methylation

Ahlert, D., Stegemann, S., Kahlau, S., Ruf, S., & Bock, R. (2009). Insensitivity of chloroplast gene expression to DNA methylation. Molecular Genetics and Genomics, 282(1), 17-24. doi:10.1007/s00438-009-0440-z.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2646-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2647-0
Genre: Journal Article

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 Creators:
Ahlert, D.1, Author
Stegemann, S.2, Author              
Kahlau, S.2, Author              
Ruf, S.2, Author              
Bock, R.2, Author              
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1External Organizations, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: chloroplast adenine methylation cytosine methylation dam methylation plastid transformation nicotiana tabacum tomato fruit-development transcriptional regulation chlamydomonas-reinhardtii maternal inheritance plastid genome higher-plants cytosine-methylation protein interactions tobacco transformation
 Abstract: Presence and possible functions of DNA methylation in plastid genomes of higher plants have been highly controversial. While a number of studies presented evidence for the occurrence of both cytosine and adenine methylation in plastid genomes and proposed a role of cytosine methylation in the transcriptional regulation of plastid genes, several recent studies suggested that at least cytosine methylation may be absent from higher plant plastid genomes. To test if either adenine or cytosine methylation can play a regulatory role in plastid gene expression, we have introduced cyanobacterial genes for adenine and cytosine DNA methyltransferases (methylases) into the tobacco plastid genome by chloroplast transformation. Using DNA cleavage with methylation-sensitive and methylation-dependent restriction endonucleases, we show that the plastid genomes in the transplastomic plants are efficiently methylated. All transplastomic lines are phenotypically indistinguishable from wild-type plants and, moreover, show no alterations in plastid gene expression. Our data indicate that the expression of plastid genes is not sensitive to DNA methylation and, hence, suggest that DNA methylation is unlikely to be involved in the transcriptional regulation of plastid gene expression.

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Language(s): eng - English
 Dates: 2009-03-182009
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000266950400002
DOI: 10.1007/s00438-009-0440-z
ISSN: 1617-4623 (Electronic) 1617-4623 (Linking)
URI: ://000266950400002 http://www.springerlink.com/content/5224417147504600/fulltext.pdf
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Title: Molecular Genetics and Genomics
Source Genre: Journal
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Pages: - Volume / Issue: 282 (1) Sequence Number: - Start / End Page: 17 - 24 Identifier: -