English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Insensitivity of chloroplast gene expression to DNA methylation

Ahlert, D., Stegemann, S., Kahlau, S., Ruf, S., & Bock, R. (2009). Insensitivity of chloroplast gene expression to DNA methylation. Molecular Genetics and Genomics, 282(1), 17-24. doi:10.1007/s00438-009-0440-z.

Item is

Files

show Files
hide Files
:
Ahlert-2009-Insensitivity of chl.pdf (Any fulltext), 510KB
Name:
Ahlert-2009-Insensitivity of chl.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Ahlert, D.1, Author
Stegemann, S.2, Author           
Kahlau, S.2, Author           
Ruf, S.2, Author           
Bock, R.2, Author           
Affiliations:
1External Organizations, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

Content

show
hide
Free keywords: chloroplast adenine methylation cytosine methylation dam methylation plastid transformation nicotiana tabacum tomato fruit-development transcriptional regulation chlamydomonas-reinhardtii maternal inheritance plastid genome higher-plants cytosine-methylation protein interactions tobacco transformation
 Abstract: Presence and possible functions of DNA methylation in plastid genomes of higher plants have been highly controversial. While a number of studies presented evidence for the occurrence of both cytosine and adenine methylation in plastid genomes and proposed a role of cytosine methylation in the transcriptional regulation of plastid genes, several recent studies suggested that at least cytosine methylation may be absent from higher plant plastid genomes. To test if either adenine or cytosine methylation can play a regulatory role in plastid gene expression, we have introduced cyanobacterial genes for adenine and cytosine DNA methyltransferases (methylases) into the tobacco plastid genome by chloroplast transformation. Using DNA cleavage with methylation-sensitive and methylation-dependent restriction endonucleases, we show that the plastid genomes in the transplastomic plants are efficiently methylated. All transplastomic lines are phenotypically indistinguishable from wild-type plants and, moreover, show no alterations in plastid gene expression. Our data indicate that the expression of plastid genes is not sensitive to DNA methylation and, hence, suggest that DNA methylation is unlikely to be involved in the transcriptional regulation of plastid gene expression.

Details

show
hide
Language(s): eng - English
 Dates: 2009-03-182009
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000266950400002
DOI: 10.1007/s00438-009-0440-z
ISSN: 1617-4623 (Electronic) 1617-4623 (Linking)
URI: ://000266950400002 http://www.springerlink.com/content/5224417147504600/fulltext.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Genetics and Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 282 (1) Sequence Number: - Start / End Page: 17 - 24 Identifier: -