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  Combined metabolomic and genetic approaches reveal a link between the polyamine pathway and albumin 2 in developing pea seeds

Vigeolas, H., Chinoy, C., Zuther, E., Blessington, B., Geigenberger, P., & Domoney, C. (2008). Combined metabolomic and genetic approaches reveal a link between the polyamine pathway and albumin 2 in developing pea seeds. Plant Physiology, 146(1), 74-82. doi:10.1104/pp.107.111369.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-266A-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-266B-F
Genre: Journal Article

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 Creators:
Vigeolas, H.1, Author              
Chinoy, C.2, Author
Zuther, E.3, Author              
Blessington, B.2, Author
Geigenberger, P.1, Author              
Domoney, C.2, Author
Affiliations:
1Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              
2External Organizations, ou_persistent22              
3Transcript Profiling, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753306              

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Free keywords: pisum-sativum-l fruit-development medicago-sativa potato-tubers arabidopsis proteins chromatography spermidine hemopexin sequence
 Abstract: Several legume seed proteins that are potentially allergenic, poorly digested by farm animals, and/ or have undesirable functional properties, have been described. One of these is the albumin protein in pea (Pisum sativum) called PA2. A naturally occurring mutant line that lacks PA2 has been exploited in studies to determine the biological function of this nonstorage protein in seed development. The mutant, which has a small seed, a tall plant phenotype, and lacks most of the PA2-encoding genes, has been crossed with a standard cultivar, 'Birte,' which contains PA2 to give rise to a recombinant inbred (RI) population. An F 3 line carrying the mutation and having a short plant phenotype has been used to generate backcross (BC) lines with 'Birte.' Despite having a lower albumin content, seeds from the mutant parent and RI lines lacking PA2 have an equivalent or higher seed nitrogen content. Metabolite profiling of seeds revealed major differences in amino acid composition and polyamine content in the two parent lines. This was investigated further in BC lines, where the effects of differences in seed size and plant height between the two parents were eliminated. Here, differences in polyamine synthesis were maintained as was a difference in total seed protein between the BC line lacking PA2 and 'Birte.' Analysis of enzyme activities in the pathways of polyamine synthesis revealed that the differences in spermidine content were attributable to changes in the overall activities of spermidine synthase and arginine decarboxylase. Although the genes encoding spermidine synthase and PA2 both localized to the pea linkage group I, the two loci were shown not to be closely linked and to have recombined in the BC lines. A distinct locus on linkage group III contains a gene that is related to PA2 but expressed predominantly in flowers. The results provide evidence for a role of PA2 in regulating polyamine metabolism, which has important functions in development, metabolism, and stress responses in plants.

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Language(s): eng - English
 Dates: 2007-11-212008
 Publication Status: Published in print
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 Table of Contents: -
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 Identifiers: ISI: ISI:000252093100007
DOI: 10.1104/pp.107.111369
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000252093100007 http://www.jstor.org/stable/pdfplus/40065819.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 146 (1) Sequence Number: - Start / End Page: 74 - 82 Identifier: -