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  The potato-specific apyrase is apoplastically localized and has influence on gene expression, growth, and development

Riewe, D., Grosman, L., Fernie, A. R., Wucke, C., & Geigenberger, P. (2008). The potato-specific apyrase is apoplastically localized and has influence on gene expression, growth, and development. Plant Physiology, 147(3), 1092-1109. doi:10.1104/pp.108.117564.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-26CC-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-853C-2
Genre: Journal Article

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 Creators:
Riewe, D.1, Author
Grosman, L.1, Author
Fernie, A. R.2, Author              
Wucke, C.1, Author
Geigenberger, P.3, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              

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Free keywords: Apyrase/genetics/*metabolism Cloning, Molecular Consensus Sequence Gene Expression Gene Expression Profiling Gene Expression Regulation, Plant Green Fluorescent Proteins/metabolism Plant Epidermis/enzymology Plant Leaves/enzymology Plant Tubers/*enzymology/growth & development Plants, Genetically Modified/enzymology Promoter Regions (Genetics) RNA, Messenger/metabolism Solanum tuberosum/*enzymology/genetics/growth & development
 Abstract: Apyrases hydrolyze nucleoside triphosphates and diphosphates and are found in all eukaryotes and a few prokaryotes. Although their enzymatic properties have been well characterized, relatively little is known regarding their subcellular localization and physiological function in plants. In this study, we used reverse genetic and biochemical approaches to investigate the role of potato (Solanum tuberosum)-specific apyrase. Silencing of the apyrase gene family with RNA interference constructs under the control of the constitutive 35S promoter led to a strong decrease in apyrase activity to below 10% of the wild-type level. This decreased activity led to phenotypic changes in the transgenic lines, including a general retardation in growth, an increase in tuber number per plant, and differences in tuber morphology. Silencing of apyrase under the control of a tuber-specific promoter led to similar changes in tuber morphology; however, there were no direct effects of apyrase inhibition on tuber metabolism. DNA microarrays revealed that decreased expression of apyrase leads to increased levels of transcripts coding for cell wall proteins involved in growth and genes involved in energy transfer and starch synthesis. To place these results in context, we determined the subcellular localization of the potato-specific apyrase. Using a combination of approaches, we were able to demonstrate that this enzyme is localized to the apoplast. We describe the evidence that underlies both this fact and that potato-specific apyrase has a crucial role in regulating growth and development.

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Language(s): eng - English
 Dates: 2008-05-162008
 Publication Status: Published in print
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 147 (3) Sequence Number: - Start / End Page: 1092 - 1109 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438