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  Identification and Characterization of ADNT1, a Novel Mitochondrial Adenine Nucleotide Transporter from Arabidopsis

Palmieri, L., Santoro, A., Carrari, F., Blanco, E., Nunes-Nesi, A., Arrigoni, R., et al. (2008). Identification and Characterization of ADNT1, a Novel Mitochondrial Adenine Nucleotide Transporter from Arabidopsis. Plant Physiology, 148(4), 1797-1808. doi:10.1104/pp.108.130310.

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Palmieri, L.1, Author
Santoro, A.1, Author
Carrari, F.2, Author              
Blanco, E.1, Author
Nunes-Nesi, A.2, Author              
Arrigoni, R.1, Author
Genchi, F.1, Author
Fernie, A. R.2, Author              
Palmieri, F.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: functional-characterization bacterial expression saccharomyces-cerevisiae plant-mitochondria tissue distribution electron-transport uncoupling protein molecular-identification thiamine pyrophosphate bovine mitochondria
 Abstract: Despite the fundamental importance and high level of compartmentation of mitochondrial nucleotide metabolism in plants, our knowledge concerning the transport of nucleotides across intracellular membranes remains far from complete. Study of a previously uncharacterized Arabidopsis (Arabidopsis thaliana) gene (At4g01100) revealed it to be a novel adenine nucleotide transporter, designated ADNT1, belonging to the mitochondrial carrier family. The ADNT1 gene shows broad expression at the organ level. Green fluorescent protein-based cell biological analysis demonstrated targeting of ADNT1 to mitochondria. While analysis of the expression of b-glucuronidase fusion proteins suggested that it was expressed across a broad range of tissue types, it was most highly expressed in root tips. Direct transport assays with recombinant and reconstituted ADNT1 were utilized to demonstrate that this protein displays a relatively narrow substrate specificity largely confined to adenylates and their closest analogs. ATP uptake was markedly inhibited by the presence of other adenylates and general inhibitors of mitochondrial transport but not by bongkrekate or carboxyatractyloside, inhibitors of the previously characterized ADP/ATP carrier. Furthermore, the kinetics are substantially different from those of this carrier, with ADNT1 preferring AMP to ADP. Finally, isolation and characterization of a T-DNA insertional knockout mutant of ADNT1, alongside complementation and antisense approaches, demonstrated that although deficiency of this transporter did not seem to greatly alter photosynthetic metabolism, it did result in reduced root growth and respiration. These findings are discussed in the context of a potential function for ADNT1 in the provision of the energy required to support growth in heterotrophic plant tissues.

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Language(s): eng - English
 Dates: 2008-10-172008
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: ISI: ISI:000261501500007
DOI: 10.1104/pp.108.130310
ISSN: 0032-0889 (Print)0032-0889 (Linking)
URI: ://000261501500007http://www.jstor.org/stable/pdfplus/40066310.pdf
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 148 (4) Sequence Number: - Start / End Page: 1797 - 1808 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438