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  Decreased expression of plastidial adenylate kinase in potato tubers results in an enhanced rate of respiration and a stimulation of starch synthesis that is attributable to post-translational redox-activation of ADP-glucose pyrophosphorylase

Oliver, S. N., Tiessen, A., Fernie, A. R., & Geigenberger, P. (2008). Decreased expression of plastidial adenylate kinase in potato tubers results in an enhanced rate of respiration and a stimulation of starch synthesis that is attributable to post-translational redox-activation of ADP-glucose pyrophosphorylase. Journal of Experimental Botany, 59(2), 315-325. doi:10.1093/Jxb/Erm312.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-26DE-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8536-E
Genre: Journal Article

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 Creators:
Oliver, S. N.1, Author              
Tiessen, A.1, Author              
Fernie, A. R.2, Author              
Geigenberger, P.1, Author              
Affiliations:
1Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: adenylate kinase adpglucose pyrophosphorylase plastid redox-regulation potato respiration starch metabolic-control analysis adpglucose pyrophosphorylase sucrose degradation transgenic plants glycolytic-flux organic-acids amino-acids leads sugars nucleotides
 Abstract: Adenine nucleotides are of general importance for many aspects of cell function, but their role in the regulation of biosynthetic processes is still unclear. It was previously reported that decreased expression of plastidial adenylate kinase, catalysing the interconversion of ATP and AMP to ADP, leads to increased adenylate pools and starch content in transgenic potato tubers. However, the underlying mechanisms were not elucidated. Here, it is shown that decreased expression of plastidial adenylate kinase in growing tubers leads to increased rates of respiratory oxygen consumption and increased carbon fluxes into starch. Increased rates of starch synthesis were accompanied by post-translational redox-activation of ADP-glucose pyrophosphorylase (AGPase), catalysing the key regulatory step of starch synthesis in the plastid, while there were no substantial changes in metabolic intermediates or sugar levels. A similar increase in post-translational redox-activation of AGPase was found after supplying adenine to wild-type potato tuber discs to increase adenine nucleotide levels. Results provide first evidence for a link between redox-activation of AGPase and adenine nucleotide levels in plants.

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Language(s): eng - English
 Dates: 2008-02-072008
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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 Identifiers: ISI: ISI:000254006500018
DOI: 10.1093/Jxb/Erm312
ISSN: 1460-2431 (Electronic)0022-0957 (Linking)
URI: ://000254006500018http://jxb.oxfordjournals.org/content/59/2/315.full.pdf
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 59 (2) Sequence Number: - Start / End Page: 315 - 325 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883