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  Decreased Expression of Cytosolic Pyruvate Kinase in Potato Tubers Leads to a Decline in Pyruvate Resulting in an in Vivo Repression of the Alternative Oxidase

Oliver, S. N., Lunn, J. E., Urbanczyk-Wochniak, E., Lytovchenko, A., van Dongen, J. T., Faix, B., et al. (2008). Decreased Expression of Cytosolic Pyruvate Kinase in Potato Tubers Leads to a Decline in Pyruvate Resulting in an in Vivo Repression of the Alternative Oxidase. Plant Physiology, 148(3), 1640-1654. doi:10.1104/pp.108.126516.

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 Creators:
Oliver, S. N.1, Author              
Lunn, J. E.2, Author              
Urbanczyk-Wochniak, E.3, Author              
Lytovchenko, A.3, Author              
van Dongen, J. T.4, Author              
Faix, B.5, Author
Schmalzlin, E.5, Author
Fernie, A. R.3, Author              
Geigenberger, P.1, Author              
Affiliations:
1Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              
2System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
4Energy Metabolism, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753325              
5External Organizations, ou_persistent22              

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Free keywords: higher-plant mitochondria transgenic tobacco plants low internal oxygen nad malic enzyme sucrose synthase phosphoenolpyruvate carboxylase nitrogen assimilation regulatory properties arabidopsis-thaliana malate-dehydrogenase
 Abstract: The aim of this work was to investigate the effect of decreased cytosolic pyruvate kinase (PKc) on potato (Solanum tuberosum) tuber metabolism. Transgenic potato plants with strongly reduced levels of PKc were generated by RNA interference gene silencing under the control of a tuber-specific promoter. Metabolite profiling showed that decreased PKc activity led to a decrease in the levels of pyruvate and some other organic acids involved in the tricarboxylic acid cycle. Flux analysis showed that this was accompanied by changes in carbon partitioning, with carbon flux being diverted from glycolysis toward starch synthesis. However, this metabolic shift was relatively small and hence did not result in enhanced starch levels in the tubers. Although total respiration rates and the ATP to ADP ratio were largely unchanged, transgenic tubers showed a strong decrease in the levels of alternative oxidase (AOX) protein and a corresponding decrease in the capacity of the alternative pathway of respiration. External feeding of pyruvate to tuber tissue or isolated mitochondria resulted in activation of the AOX pathway, both in the wild type and the PKc transgenic lines, providing direct evidence for the regulation of AOX by changes in pyruvate levels. Overall, these results provide evidence for a crucial role of PKc in the regulation of pyruvate levels as well as the level of the AOX in heterotrophic plant tissue, and furthermore reveal that these parameters are interlinked in vivo.

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Language(s): eng - English
 Dates: 2008-10-032008
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000260719500037
DOI: 10.1104/pp.108.126516
ISSN: 0032-0889 (Print)0032-0889 (Linking)
URI: ://000260719500037http://www.jstor.org/stable/pdfplus/40066292.pdf
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 148 (3) Sequence Number: - Start / End Page: 1640 - 1654 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438