English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli

Neupert, J., Karcher, D., & Bock, R. (2008). Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research, 36(19), e124. doi:10.1093/nar/gkn545.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-26EF-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-26F0-3
Genre: Journal Article

Files

show Files
hide Files
:
Neupert-2008-Design of simple syn.pdf (Any fulltext), 4MB
Name:
Neupert-2008-Design of simple syn.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Neupert, J.1, Author              
Karcher, D.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

Content

show
hide
Free keywords: thermosensor controls expression translation transformation riboswitches
 Abstract: RNA thermometers are thermosensors that regulate gene expression by temperature-induced changes in RNA conformation. Naturally occurring RNA thermometers exhibit complex secondary structures which are believed to undergo a series of gradual structural changes in response to temperature shifts. Here, we report the de novo design of considerably simpler RNA thermometers that provide useful RNA-only tools to regulate bacterial gene expression by a shift in the growth temperature. We show that a single small stem-loop structure containing the ribosome binding site is sufficient to construct synthetic RNA thermometers that work efficiently at physiological temperatures. Our data suggest that the thermometers function by a simple melting mechanism and thus provide minimum size on/off switches to experimentally induce or repress gene expression by temperature.

Details

show
hide
Language(s): eng - English
 Dates: 2008-08-302008
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000260983000003
DOI: 10.1093/nar/gkn545
ISSN: 1362-4962 (Electronic) 0305-1048 (Linking)
URI: ://000260983000003 http://nar.oxfordjournals.org/content/36/19/e124 http://nar.oxfordjournals.org/content/36/19/e124.full.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nucleic Acids Research
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 36 (19) Sequence Number: - Start / End Page: e124 Identifier: -