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  Natural variation in CBF gene sequence, gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana

McKhann, H. I., Gery, C., Berard, A., Leveque, S., Zuther, E., Hincha, D. K., et al. (2008). Natural variation in CBF gene sequence, gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana. BMC Plant Biology, 8, 105. doi:10.1186/1471-2229-8-105.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2707-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2708-7
Genre: Journal Article

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 Creators:
McKhann, H. I.1, Author
Gery, C.1, Author
Berard, A.1, Author
Leveque, S.1, Author
Zuther, E.2, Author              
Hincha, D. K.2, Author              
De Mita, S.1, Author
Brunel, D.1, Author
Teoule, E.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Transcript Profiling, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753306              

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Free keywords: cold-response pathway low-temperature transcription factors duplicated genes brassica-napus acclimation drought accessions regulator elements
 Abstract: Background: Plants from temperate regions are able to withstand freezing temperatures due to a process known as cold acclimation, which is a prior exposure to low, but non-freezing temperatures. During acclimation, a large number of genes are induced, bringing about biochemical changes in the plant, thought to be responsible for the subsequent increase in freezing tolerance. Key regulatory proteins in this process are the CBF1, 2 and 3 transcription factors which control the expression of a set of target genes referred to as the "CBF regulon". Results: To assess the role of the CBF genes in cold acclimation and freezing tolerance of Arabidopsis thaliana, the CBF genes and their promoters were sequenced in the Versailles core collection, a set of 48 accessions that maximizes the naturally-occurring genetic diversity, as well as in the commonly used accessions Col-0 and WS. Extensive polymorphism was found in all three genes. Freezing tolerance was measured in all accessions to assess the variability in acclimated freezing tolerance. The effect of sequence polymorphism was investigated by evaluating the kinetics of CBF gene expression, as well as that of a subset of the target COR genes, in a set of eight accessions with contrasting freezing tolerance. Our data indicate that CBF genes as well as the selected COR genes are cold induced in all accessions, irrespective of their freezing tolerance. Although we observed different levels of expression in different accessions, CBF or COR gene expression was not closely correlated with freezing tolerance. Conclusion: Our results indicate that the Versailles core collection contains significant natural variation with respect to freezing tolerance, polymorphism in the CBF genes and CBF and COR gene expression. Although there tends to be more CBF and COR gene expression in tolerant accessions, there are exceptions, reinforcing the idea that a complex network of genes is involved in freezing tolerance and that the CBF genes alone cannot explain all differences in phenotype. Our study also highlights the difficulty in assessing the function of single transcription factors that are members of closely related gene families.

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Language(s): eng - English
 Dates: 2008-10-152008
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000260828400001
DOI: 10.1186/1471-2229-8-105
ISSN: 1471-2229 (Electronic) 1471-2229 (Linking)
URI: ://000260828400001 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2579297/pdf/1471-2229-8-105.pdf?tool=pmcentrez
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Title: BMC Plant Biology
Source Genre: Journal
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Pages: - Volume / Issue: 8 Sequence Number: - Start / End Page: 105 Identifier: -