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  Analysis of expressed sequence tags from Actinidia: Applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

Crowhurst, R. N., Gleave, A. P., MacRae, E. A., Ampomah-Dwamena, C., Atkinson, R. G., Beuning, L. L., et al. (2008). Analysis of expressed sequence tags from Actinidia: Applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening. BMC Genomics, 9, 351. doi:10.1186/1471-2164-9-351.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-27DB-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-27DC-B
Genre: Journal Article

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Crowhurst, R. N.1, Author
Gleave, A. P.1, Author
MacRae, E. A.1, Author
Ampomah-Dwamena, C.1, Author
Atkinson, R. G.1, Author
Beuning, L. L.1, Author
Bulley, S. M.1, Author
Chagne, D.1, Author
Marsh, K. B.1, Author
Matich, A. J.1, Author
Montefiori, M.1, Author
Newcomb, R. D.1, Author
Schaffer, R. J.1, Author
Usadel, B.2, 3, Author              
Allan, A. C.1, Author
Boldingh, H. L.1, Author
Bowen, J. H.1, Author
Davy, M. W.1, Author
Eckloff, R.1, Author
Ferguson, A. R.1, Author
Fraser, L. G.1, AuthorGera, E.1, AuthorHellens, R. P.1, AuthorJanssen, B. J.1, AuthorKlages, K.1, AuthorLo, K. R.1, AuthorMacDiarmid, R. M.1, AuthorNain, B.1, AuthorMcNeilage, M. A.1, AuthorRassam, M.1, AuthorRichardson, A. C.1, AuthorRikkerink, E. H. A.1, AuthorRoss, G. S.1, AuthorSchroeder, R.1, AuthorSnowden, K. C.1, AuthorSouleyre, E. J. F.1, AuthorTempleton, M. D.1, AuthorWalton, E. F.1, AuthorWang, D.1, AuthorWang, M. Y.1, AuthorWang, Y. Y.1, AuthorWood, M.1, AuthorWu, R. M.1, AuthorYauk, Y. K.1, AuthorLaing, W. A.1, Author more..
Affiliations:
1External Organizations, ou_persistent22              
2Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              
3System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              

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Free keywords: kiwi-fruit biochemical-characterization cell-wall shikimate dehydrogenase microsatellite markers arabidopsis-thaliana beta-galactosidase cysteine protease fleshed kiwifruit storage proteins
 Abstract: Background: Kiwifruit (Actinidia spp.) are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by the development of new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags ( ESTs). Results: The ESTs were derived mainly from four Actinidia species ( A. chinensis, A. deliciosa, A. arguta and A. eriantha) and fell into 41,858 non redundant clusters ( 18,070 tentative consensus sequences and 23,788 EST singletons). Analysis of flavor and fragrance-related gene families ( acyltransferases and carboxylesterases) and pathways (terpenoid biosynthesis) is presented in comparison with a chemical analysis of the compounds present in Actinidia including esters, acids, alcohols and terpenes. ESTs are identified for most genes in color pathways controlling chlorophyll degradation and carotenoid biosynthesis. In the health area, data are presented on the ESTs involved in ascorbic acid and quinic acid biosynthesis showing not only that genes for many of the steps in these pathways are represented in the database, but that genes encoding some critical steps are absent. In the convenience area, genes related to different stages of fruit softening are identified. Conclusion: This large EST resource will allow researchers to undertake the tremendous challenge of understanding the molecular basis of genetic diversity in the Actinidia genus as well as provide an EST resource for comparative fruit genomics. The various bioinformatics analyses we have undertaken demonstrates the extent of coverage of ESTs for genes encoding different biochemical pathways in Actinidia.

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Language(s): eng - English
 Dates: 2008-07-272008
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000258552400001
DOI: 10.1186/1471-2164-9-351
ISSN: 1471-2164 (Electronic) 1471-2164 (Linking)
URI: ://000258552400001 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2515324/pdf/1471-2164-9-351.pdf?tool=pmcentrez
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Title: BMC Genomics
Source Genre: Journal
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Pages: - Volume / Issue: 9 Sequence Number: - Start / End Page: 351 Identifier: -