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  A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit

Bermudez, L., Urias, U., Milstein, D., Kamenetzky, L., Asis, R., Fernie, A. R., et al. (2008). A candidate gene survey of quantitative trait loci affecting chemical composition in tomato fruit. Journal of Experimental Botany, 59(10), 2875-2890. doi:10.1093/jxb/ern146.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2809-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-850B-1
Genre: Journal Article

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 Creators:
Bermudez, L.1, Author
Urias, U.1, Author
Milstein, D.1, Author
Kamenetzky, L.1, Author
Asis, R.1, Author
Fernie, A. R.2, Author              
van Sluys, M. A.1, Author
Carrari, F.2, Author              
Rossi, M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: candidate genes introgressed lines metabolite content quantitative trait loci solanum lycopersicum solanum pennelli tomato carbohydrate-metabolism introgression line sequence qtls map biosynthesis transcript methionine expression evolution
 Abstract: In tomato, numerous wild-related species have been demonstrated to be untapped sources of valuable genetic variability, including pathogen-resistance genes, nutritional, and industrial quality traits. From a collection of S. pennellii introgressed lines, 889 fruit metabolic loci (QML) and 326 yield-associated loci (YAL), distributed across the tomato genome, had been identified previously. By using a combination of molecular marker sequence analysis, PCR amplification and sequencing, analysis of allelic variation, and evaluation of co-response between gene expression and metabolite composition traits, the present report, provides a comprehensive list of candidate genes co-localizing with a subset of 106 QML and 20 YAL associated either with important agronomic or nutritional characteristics. This combined strategy allowed the identification and analysis of 127 candidate genes located in 16 regions of the tomato genome. Eighty-five genes were cloned and partially sequenced, totalling 45 816 and 45 787 bases from S. lycopersicum and S. pennellii , respectively. Allelic variation at the amino acid level was confirmed for 37 of these candidates. Furthermore, out of the 127 gene-metabolite co-locations, some 56 were recovered following correlation of parallel transcript and metabolite profiling. Results obtained here represent the initial steps in the integration of genetic, genomic, and expressional patterns of genes co-localizing with chemical compositional traits of the tomato fruit.

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Language(s): eng - English
 Dates: 2008-06-172008
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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 Identifiers: ISI: ISI:000257401500025
DOI: 10.1093/jxb/ern146
ISSN: 1460-2431 (Electronic)0022-0957 (Linking)
URI: ://000257401500025http://jxb.oxfordjournals.org/content/59/10/2875.full.pdf
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 59 (10) Sequence Number: - Start / End Page: 2875 - 2890 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883