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  Profiling diurnal changes in metabolite and transcript levels in potato leaves

Urbanczyk-Wochniak, E., Baxter, C., Sweetlove, L. J., & Fernie, A. R. (2007). Profiling diurnal changes in metabolite and transcript levels in potato leaves. In 3rd International Congress on Plant Metabolomics (pp. 183-192).

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Urbanczyk-Wochniak, E.1, Author              
Baxter, C.2, Author
Sweetlove, L. J.2, Author
Fernie, A. R.1, Author              
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1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              

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Free keywords: alpha-ketoglutarate aminotransferase nitrate reductase-activity tobacco plants deficient phosphoenolpyruvate carboxylase isocitrate dehydrogenase glutamate-dehydrogenase transgenic alfalfa carbon metabolism acid-metabolism gene-expression
 Abstract: The availability of sequence data is contributing immensely to the development of gene-expression resources, in parallel to these advances, several methods have been established for systematic analysis of metabolite composition. In this chapter, we illustrate the utility of parallel transcript and metabolic profiling analysis to study metabolic regulation during the day/night cycle. Recently we presented a gas chromatography-mass spectrometry-based metabolic profiling protocol, alongside spectrophotometric techniques, to follow changes in a broad range of potato leaf metabolites throughout the day/night cycle (Urbanczyk-Wochniak et al., 2005). In tandem, we profiled transcript levels using both a custom array containing approximately 2,500 cDNA clones representing predominantly transcripts involved in plant metabolism, and commercially available arrays containing approximately 12,000 cDNA clones that gave coverage of transcript levels over a broader functional range. The levels of many metabolites and transcripts varied during the day/night cycle. Whilst a large number of the differences might be expected based on earlier data, several novel changes were seen. Here we present novel description of changes in metabolites and genes associated with secondary metabolism. Profiling of diurnal patterns of metabolite and transcript abundance in potato leaves suggests that specific sets of metabolic pathway are strongly transcriptionally regulated, but revealed that the majority of the metabolic network is primarily under post-transcriptional control.

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Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: -
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 Identifiers: ISI: ISI:000249562000013
DOI: 10.1007/978-1-4020-5608-6_13
URI: ://000249562000013 http://link.springer.com/content/pdf/10.1007%2F978-1-4020-5608-6_13
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Title: 3rd International Congress on Plant Metabolomics
Place of Event: Iowa State Univ, Ames, IA
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Title: 3rd International Congress on Plant Metabolomics
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 183 - 192 Identifier: -