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  Deficiency in phosphatidylserine decarboxylase activity in the psd1 psd2 psd3 triple mutant of Arabidopsis affects phosphatidylethanolamine accumulation in mitochondria

Nerlich, A., von Orlow, M., Rontein, D., Hanson, A. D., & Doermann, P. (2007). Deficiency in phosphatidylserine decarboxylase activity in the psd1 psd2 psd3 triple mutant of Arabidopsis affects phosphatidylethanolamine accumulation in mitochondria. Plant Physiology, 144(2), 904-914. doi:10.1104/pp.107.095414.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-28B7-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-28B8-F
Genre: Journal Article

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 Creators:
Nerlich, A.1, Author
von Orlow, M.1, Author
Rontein, D.1, Author
Hanson, A. D.1, Author
Doermann, P.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Plant Lipids, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753347              

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Free keywords: Amino Acid Sequence Arabidopsis/*enzymology/genetics/metabolism Carboxy-Lyases/genetics/isolation & purification/*metabolism Gene Expression Mitochondria/*metabolism Molecular Sequence Data Mutation, Missense Phosphatidylethanolamines/*biosynthesis Phospholipids/*metabolism
 Abstract: Phosphatidylserine (PS) decarboxylase is involved in the synthesis of the abundant phospholipid phosphatidylethanolamine (PE), particularly in mitochondria, in many organisms, including yeast (Saccharomyces cerevisiae) and animals. Arabidopsis (Arabidopsis thaliana) contains three genes with sequence similarity to PS decarboxylases, and the respective gene products were functionally characterized after heterologous expression in yeast and Escherichia coli. While the PSD1 protein localizes to mitochondria, PSD2 and PSD3 are found in the endomembrane system. To study the role of PSD genes in plant phospholipid metabolism, Arabidopsis insertional mutants for psd1, psd2, and psd3 were obtained. The single mutants were decreased in PS decarboxylase activity to various extents, but mutant plants showed no obvious growth or morphological phenotype. A triple mutant, psd1 psd2 psd3, was generated that was totally devoid of PS decarboxylase activity. While the phospholipid composition in whole leaves was unchanged, the PE content in isolated mitochondria of psd1 psd2 psd3 was decreased. Therefore, the predominant proportion of PE in Arabidopsis is synthesized by alternative pathways, but a significant amount of mitochondrial PE is derived from the PS decarboxylase reaction. These results imply that, similar to yeast and animal cells, a specific phospholipid transfer from the endoplasmic reticulum to mitochondria exists in plants.

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Language(s): eng - English
 Dates: 2007-04-242007
 Publication Status: Published in print
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 144 (2) Sequence Number: - Start / End Page: 904 - 914 Identifier: -