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  Medicago truncatula root nodule proteome analysis reveals differential plant and bacteroid responses to drought stress

Larrainzar, E., Wienkoop, S., Weckwerth, W., Ladrera, R., Arrese-Igor, C., & Gonzalez, E. M. (2007). Medicago truncatula root nodule proteome analysis reveals differential plant and bacteroid responses to drought stress. Plant Physiology, 144(3), 1495-1507. doi:10.1104/pp.107.101618.

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Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-28E3-D Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-28E4-B
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Larrainzar, E.1, Autor
Wienkoop, S.2, Autor              
Weckwerth, W.2, Autor              
Ladrera, R.1, Autor
Arrese-Igor, C.1, Autor
Gonzalez, E. M.1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Integrative Proteomics and Metabolomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753334              

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Schlagwörter: quantitative shotgun proteomics symbiotic nitrogen-fixation sucrose synthase activity sinorhizobium-meliloti asparagine synthetase soybean nodules n-2 fixation 2-dimensional electrophoresis aphanomyces-euteiches methionine metabolism
 Zusammenfassung: Drought is one of the environmental factors most affecting crop production. Under drought, symbiotic nitrogen fixation is one of the physiological processes to first show stress responses in nodulated legumes. This inhibition process involves a number of factors whose interactions are not yet understood. This work aims to further understand changes occurring in nodules under drought stress from a proteomic perspective. Drought was imposed on Medicago truncatula 'Jemalong A17' plants grown in symbiosis with Sinorhizobium meliloti strain 2011. Changes at the protein level were analyzed using a nongel approach based on liquid chromatography coupled to tandem mass spectrometry. Due to the complexity of nodule tissue, the separation of plant and bacteroid fractions in M. truncatula root nodules was first checked with the aim of minimizing cross contamination between the fractions. Second, the protein plant fraction of M. truncatula nodules was profiled, leading to the identification of 377 plant proteins, the largest description of the plant nodule proteome so far. Third, both symbiotic partners were independently analyzed for quantitative differences at the protein level during drought stress. Multivariate data mining allowed for the classification of proteins sets that were involved in drought stress responses. The isolation of the nodule plant and bacteroid protein fractions enabled the independent analysis of the response of both counterparts, gaining further understanding of how each symbiotic member is distinctly affected at the protein level under a water-deficit situation.

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Sprache(n): eng - Englisch
 Datum: 2007-06-052007
 Publikationsstatus: Im Druck publiziert
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 Identifikatoren: ISI: ISI:000247849400024
DOI: 10.1104/pp.107.101618
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000247849400024 http://www.jstor.org/stable/pdfplus/40065658.pdf
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Titel: Plant Physiology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 144 (3) Artikelnummer: - Start- / Endseite: 1495 - 1507 Identifikator: -