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  Phosphorus stress in common bean: Root transcript and metabolic responses

Hernandez, G., Ramirez, M., Valdes-Lopez, O., Tesfaye, M., Graham, M. A., Czechowski, T., et al. (2007). Phosphorus stress in common bean: Root transcript and metabolic responses. Plant Physiology, 144(2), 752-767. doi:10.1104/pp.107.096958.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-291C-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-291D-3
Genre: Journal Article

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 Creators:
Hernandez, G.1, Author
Ramirez, M.1, Author
Valdes-Lopez, O.1, Author
Tesfaye, M.1, Author
Graham, M. A.1, Author
Czechowski, T.1, Author
Schlereth, A.2, Author              
Wandrey, M.2, Author              
Erban, A.3, Author              
Cheung, F.1, Author
Wu, H. C.1, Author
Lara, M.1, Author
Town, C. D.1, Author
Kopka, J.3, Author              
Udvardi, M. K.2, Author              
Vance, C. P.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Molecular Plant Nutrition, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753321              
3Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

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Free keywords: symbiotic nitrogen-fixation organic-acid exudation phosphate-starvation arabidopsis-thaliana gene-expression system architecture phaseolus-vulgaris lotus-japonicus proteoid roots white lupin
 Abstract: Phosphorus (P) is an essential element for plant growth. Crop production of common bean (Phaseolus vulgaris), the most important legume for human consumption, is often limited by low P in the soil. Functional genomics were used to investigate global gene expression and metabolic responses of bean plants grown under P-deficient and P-sufficient conditions. P-deficient plants showed enhanced root to shoot ratio accompanied by reduced leaf area and net photosynthesis rates. Transcript profiling was performed through hybridization of nylon filter arrays spotted with cDNAs of 2,212 unigenes from a P deficiency root cDNA library. A total of 126 genes, representing different functional categories, showed significant differential expression in response to P: 62% of these were induced in P-deficient roots. A set of 372 bean transcription factor (TF) genes, coding for proteins with Inter-Pro domains characteristic or diagnostic for TF, were identified from The Institute of Genomic Research/ Dana Farber Cancer Institute Common Bean Gene Index. Using real-time reverse transcription-polymerase chain reaction analysis, 17 TF genes were differentially expressed in P-deficient roots; four TF genes, including MYB TFs, were induced. Nonbiased metabolite profiling was used to assess the degree to which changes in gene expression in P-deficient roots affect overall metabolism. Stress-related metabolites such as polyols accumulated in P-deficient roots as well as sugars, which are known to be essential for P stress gene induction. Candidate genes have been identified that may contribute to root adaptation to P deficiency and be useful for improvement of common bean.

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Language(s): eng - English
 Dates: 2007-04-242007
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000247075000022
DOI: 10.1104/pp.107.096958
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000247075000022 http://www.jstor.org/stable/pdfplus/40065527.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 144 (2) Sequence Number: - Start / End Page: 752 - 767 Identifier: -