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  The COMATOSE ATP-binding cassette transporter is required for full fertility in arabidopsis

Footitt, S., Dietrich, D., Fait, A., Fernie, A. R., Holdsworth, M. J., Baker, A., et al. (2007). The COMATOSE ATP-binding cassette transporter is required for full fertility in arabidopsis. Plant Physiology, 144(3), 1467-1480. doi:10.1104/pp.107.099903.

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 Creators:
Footitt, S.1, Author
Dietrich, D.1, Author
Fait, A.2, Author              
Fernie, A. R.2, Author              
Holdsworth, M. J.1, Author
Baker, A.1, Author
Theodoulou, F. L.1, Author
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1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: acid beta-oxidation pollen-tube growth male gametophyte development male-sterile mutant fatty-acid monosaccharide transporter female gametophyte flower development seed-germination glyoxylate cycle
 Abstract: COMATOSE (CTS) encodes a peroxisomal ATP-binding cassette transporter required not only for beta-oxidation of storage lipids during germination and establishment, but also for biosynthesis of jasmonic acid and conversion of indole butyric acid to indole acetic acid. cts mutants exhibited reduced fertilization, which was rescued by genetic complementation, but not by exogenous application of jasmonic acid or indole acetic acid. Reduced fertilization was also observed in thiolase (kat2-1) and peroxisomal acyl-Coenzyme A synthetase mutants (lacs6-1, lacs7-1), indicating a general role for beta-oxidation in fertility. Genetic analysis revealed reduced male transmission of cts alleles and both cts pollen germination and tube growth in vitro were impaired in the absence of an exogenous carbon source. Aniline blue staining of pollinated pistils demonstrated that pollen tube growth was affected only when both parents bore the cts mutation, indicating that expression of CTS in either male or female tissues was sufficient to support pollen tube growth in vivo. Accordingly, abundant peroxisomes were detected in a range of maternal tissues. Although gamma-aminobutyric acid levels were reduced in flowers of cts mutants, they were unchanged in kat2-1, suggesting that alterations in gamma-aminobutyric acid catabolism do not contribute to the reduced fertility phenotype through altered pollen tube targeting. Taken together, our data support an important role for beta-oxidation in fertility in Arabidopsis (Arabidopsis thaliana) and suggest that this pathway could play a role in the mobilization of lipids in both pollen and female tissues.

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Language(s): eng - English
 Dates: 2007-05-012007
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000247849400022
DOI: 10.1104/pp.107.099903
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000247849400022 http://www.jstor.org/stable/pdfplus/40065656.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 144 (3) Sequence Number: - Start / End Page: 1467 - 1480 Identifier: -