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  Deletion of glycine decarboxylase in arabidopsis is lethal under nonphotorespiratory conditions

Engel, N., van den Daele, K., Kolukisaoglu, U., Morgenthal, K., Weckwerth, W., Parnik, T., et al. (2007). Deletion of glycine decarboxylase in arabidopsis is lethal under nonphotorespiratory conditions. Plant Physiology, 144(3), 1328-1335. doi:10.1104/pp.107.099317.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2941-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2942-D
Genre: Journal Article

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 Creators:
Engel, N.1, Author
van den Daele, K.1, Author
Kolukisaoglu, U.1, Author
Morgenthal, K.1, Author
Weckwerth, W.2, Author              
Parnik, T.1, Author
Keerberg, O.1, Author
Bauwe, H.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Integrative Proteomics and Metabolomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753334              

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Free keywords: pea leaf mitochondria one-carbon metabolism serine hydroxymethyltransferase thaliana plants mutants photorespiration complex infiltration expression glyoxylate
 Abstract: The mitochondrial multienzyme glycine decarboxylase (GDC) catalyzes the tetrahydrofolate-dependent catabolism of glycine to 5,10-methylene-tetrahydrofolate and the side products NADH, CO 2, and NH3. This reaction forms part of the photorespiratory cycle and contributes to one-carbon metabolism. While the important role of GDC for these two metabolic pathways is well established, the existence of bypassing reactions has also been suggested. Therefore, it is not clear to what extent GDC is obligatory for these processes. Here, we report on features of individual and combined T-DNA insertion mutants for one of the GDC subunits, P protein, which is encoded by two genes in Arabidopsis (Arabidopsis thaliana). The individual knockout of either of these two genes does not significantly alter metabolism and photosynthetic performance indicating functional redundancy. In contrast, the double mutant does not develop beyond the cotyledon stage in air enriched with 0.9% CO2. Rosette leaves do not appear and the seedlings do not survive for longer than about 3 to 4 weeks under these nonphotorespiratory conditions. This feature distinguishes the GDC-lacking double mutant from all other known photorespiratory mutants and provides evidence for the nonreplaceable function of GDC in vital metabolic processes other than photorespiration.

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Language(s): eng - English
 Dates: 2007-05-152007
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000247849400010
DOI: 10.1104/pp.107.099317
ISSN: 0032-0889 (Print) 0032-0889 (Linking)
URI: ://000247849400010 http://www.jstor.org/stable/pdfplus/40065644.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 144 (3) Sequence Number: - Start / End Page: 1328 - 1335 Identifier: -