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  Tobacco plastid ribosomal protein S18 is essential for cell survival

Rogalski, M., Ruf, S., & Bock, R. (2006). Tobacco plastid ribosomal protein S18 is essential for cell survival. Nucleic Acids Research, 34(16), 4537-4545. doi:10.1093/nar/gkl634.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-29E0-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-29E1-7
Genre: Journal Article

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Rogalski, M.1, Author              
Ruf, S.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: chloroplast genome higher-plants deficient plastids gene DNA transformation evolution sequence subunit nucleus
 Abstract: Plastid genomes contain a conserved set of genes most of which are involved in either photosynthesis or gene expression. Among the ribosomal protein genes present in higher plant plastid genomes, rps18 is special in that it is absent from the plastid genomes of several non-green unicellular organisms, including Euglena longa and Toxoplasma gondii. Here we have tested whether the ribosomal protein S18 is required for translation by deleting the rps18 gene from the tobacco plastid genome. We report that, while deletion of the rps18 gene was readily obtained, no homoplasmic Delta rps18 plants or leaf sectors could be isolated. Instead, segregation into homoplasmy led to severe defects in leaf development suggesting that the knockout of rps18 is lethal and the S18 protein is required for cell survival. Our data demonstrate that S18 is indispensable for plastid ribosome function in tobacco and support an essential role for plastid translation in plant development. Moreover, we demonstrate the occurrence of flip-flop recombination on short inverted repeat sequences which generates different isoforms of the transformed plastid genome that differ in the orientation a 70 kb segment in the large single-copy region. However, infrequent occurrence of flip-flop recombination and random segregation of plastid genomes result in the predominant presence of only one of the isoforms in many tissue samples. Implications for the interpretation of chloroplast transformation experiments and vector design are discussed.

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Language(s): eng - English
 Dates: 2006-09-022006
 Publication Status: Published in print
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 Table of Contents: -
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 Identifiers: ISI: ISI:000241277200028
DOI: 10.1093/nar/gkl634
ISSN: 1362-4962 (Electronic) 0305-1048 (Linking)
URI: ://000241277200028 http://nar.oxfordjournals.org/content/34/16/4537.full.pdf#page=1&view=FitH
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 34 (16) Sequence Number: - Start / End Page: 4537 - 4545 Identifier: -