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  Identification of small non-coding RNAs from mitochondria and chloroplasts

Lung, B., Zemann, A., Madej, M. J., Schuelke, M., Techritz, S., Ruf, S., et al. (2006). Identification of small non-coding RNAs from mitochondria and chloroplasts. Nucleic Acids Research, 34(14), 3842-3852. doi:10.1093/Nar/Gkl448.

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Lung, B.1, Autor
Zemann, A.1, Autor
Madej, M. J.1, Autor
Schuelke, M.1, Autor
Techritz, S.1, Autor
Ruf, S.2, Autor           
Bock, R.2, Autor           
Huttenhofer, A.1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Schlagwörter: non-messenger rnas cyclic electron flow gene-expression higher-plants escherichia-coli ribosomal-rna ndhb gene nonphotosynthetic plant nad(p)h dehydrogenase experimental rnomics
 Zusammenfassung: Small non-protein-coding RNAs (ncRNAs) have been identified in a wide spectrum of organisms ranging from bacteria to humans. In eukarya, systematic searches for ncRNAs have so far been restricted to the nuclear or cytosolic compartments of cells. Whether or not small stable non-coding RNA species also exist in cell organelles, in addition to tRNAs or ribosomal RNAs, is unknown. We have thus generated cDNA libraries from size-selected mammalian mitochondrial RNA and plant chloroplast RNA and searched for small ncRNA species in these two types of DNA-containing cell organelles. In total, we have identified 18 novel candidates for organellar ncRNAs in these two cellular compartments and confirmed expression of six of them by northern blot analysis or RNase A protection assays. Most candidate ncRNA genes map to intergenic regions of the organellar genomes. As found previously in bacteria, the presumptive ancestors of present-day chloroplasts and mitochondria, we also observed examples of antisense ncRNAs that potentially could target organelle-encoded mRNAs. The structural features of the identified ncRNAs as well as their possible cellular functions are discussed. The absence from our libraries of abundant small RNA species that are not encoded by the organellar genomes suggests that the import of RNAs into cell organelles is of very limited significance or does not occur at all.

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Sprache(n): eng - English
 Datum: 2006-08-112006
 Publikationsstatus: Erschienen
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 Identifikatoren: ISI: ISI:000240583800006
DOI: 10.1093/Nar/Gkl448
ISSN: 1362-4962 (Electronic) 0305-1048 (Linking)
URI: ://000240583800006 http://nar.oxfordjournals.org/content/34/14/3842.full.pdf
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Titel: Nucleic Acids Research
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 34 (14) Artikelnummer: - Start- / Endseite: 3842 - 3852 Identifikator: -