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  Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes

Gibon, Y., Usadel, B., Blaesing, O. E., Kamlage, B., Hoehne, M., Trethewey, R., et al. (2006). Integration of metabolite with transcript and enzyme activity profiling during diurnal cycles in Arabidopsis rosettes. Genome Biology, 7(8), R76. doi:10.1186/gb-2006-7-8-R76.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2A87-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2A88-B
Genre: Journal Article

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 Creators:
Gibon, Y.1, Author              
Usadel, B.1, Author              
Blaesing, O. E.2, Author
Kamlage, B.2, Author
Hoehne, M.1, Author              
Trethewey, R.3, Author              
Stitt, M.1, Author              
Affiliations:
1System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
2External Organizations, ou_persistent22              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              

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Free keywords: Amino Acids/metabolism Arabidopsis/*enzymology/genetics Carbon/metabolism Circadian Rhythm/*physiology Energy Metabolism/*physiology Gas Chromatography-Mass Spectrometry Gene Expression Profiling Gene Expression Regulation, Enzymologic/*physiology Genome, Plant/genetics/*physiology Mutation/genetics Nitrogen/metabolism Phosphoglucomutase/genetics RNA, Messenger/*metabolism
 Abstract: ABSTRACT: BACKGROUND: Genome-wide transcript profiling and analyses of enzyme activities from central carbon and nitrogen metabolism has shown that transcript levels undergo marked and rapid changes during diurnal cycles and after transfer to darkness, whereas changes of enzyme activities are smaller and delayed. In the starchless pgm mutant, where sugars are depleted every night, there are accentuated diurnal changes of transcript levels. Enzyme activities do not show larger diurnal changes; instead they shift towards the levels found in wild-type after several days of darkness. These results indicate that enzyme activities change slowly, integrating the changes of transcript levels over several diurnal cycles. RESULTS: To generalize this conclusion, 137 metabolites were profiled using GC-MS and LC-MS. Amplitudes of the diurnal changes of metabolites in pgm were (with the exception of sugars) similar or smaller than in wild-type. The average levels shifted towards those found after several days of darkness in wild-type. Examples include increased levels of many amino acids due to protein degradation, decreased levels of many fatty acids, increased tocopherol and decreased myo-inositol. Many metabolite-transcript correlations were found and the proportion of transcripts correlated with sugars increased dramatically in the starchless pgm mutant. CONCLUSION: Rapid diurnal changes of transcripts are integrated over time to generate quasi-stable changes across large sectors of metabolism. The slow response of enzyme activities and metabolites implies that correlations between metabolites and transcripts are due to regulation of gene expression by metabolites, rather than metabolites being changed as a consequence of a change in gene expression.

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Language(s): eng - English
 Dates: 2006-08-192006
 Publication Status: Published in print
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Title: Genome Biology
Source Genre: Journal
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Pages: - Volume / Issue: 7 (8) Sequence Number: - Start / End Page: R76 Identifier: -