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  Trehalose 6-phosphate regulates starch synthesis via posttranslational redox activation of ADP-glucose pyrophosphorylase

Kolbe, A., Tiessen, A., Schluepmann, H., Paul, M., Ulrich, S., & Geigenberger, P. (2005). Trehalose 6-phosphate regulates starch synthesis via posttranslational redox activation of ADP-glucose pyrophosphorylase. Proceedings of the National Academy of Sciences of the United States of America, 102(31), 11118-11123. doi:10.1073/pnas.0503410102.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2B9A-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2B9B-8
Genre: Journal Article

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 Creators:
Kolbe, A.1, Author
Tiessen, A.2, Author              
Schluepmann, H.1, Author
Paul, M.1, Author
Ulrich, S.1, Author
Geigenberger, P.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              

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Free keywords: snf1 kinase sugar signaling thioredoxin thioredoxin-interacting protein potato-tubers adpglucose pyrophosphorylase saccharomyces-cerevisiae arabidopsis-thaliana reductive activation chloroplast enzymes subunit structure sucrose trehalose-6-phosphate
 Abstract: Trehalose is the most widespread disaccharide in nature, occurring in bacteria, fungi, insects, and plants. Its precursor, trehalose 6-phosphate (T6P), is also indispensable for the regulation of sugar utilization and growth, but the sites of action are largely unresolved. Here we use genetic and biochemical approaches to investigate whether T6P acts to regulate starch synthesis in plastids of higher plants. Feeding of trehalose to Arabidopsis leaves led to stimulation of starch synthesis within 30 min, accompanied by activation of ADP-glucose pyrophosphorylase (AGPase) via post-translational redox modification. The response resembled sucrose but not glucose feeding and depended on the expression of SNF1-related kinase. We also analyzed transgenic Arabidopsis plants with T6P levels increased by expression of T6P synthase or decreased by expression of T6P phosphatase (TPP) in the cytosol. Compared with wild type, leaves of T6P synthase-expressing plants had increased redox activation of AGPase and increased starch whereas TPP-expressing plants showed the opposite. Moreover: TPP expression prevented the increase in AGPase activation in response to sucrose or trehalose feeding. Incubation of intact isolated chloroplasts with 100 mu M T6P significantly and specifically increased reductive activation of AGPase within 15 min. Results provide evidence that T6P is synthesized in the cytosol and acts on plastidial metabolism by promoting thioredoxin-mediated redox transfer to AGPase in response to cytosolic sugar levels, thereby allowing starch synthesis to be regulated independently of light. The discovery informs about the evolution of plant metabolism and how chloroplasts of prokaryotic origin use an intermediate of the ancient trehalose pathway to report the metabolic status of the cytosol.

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Language(s): eng - English
 Dates: 2005-07-282005
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000231102400069
DOI: 10.1073/pnas.0503410102
ISSN: 0027-8424 (Print) 0027-8424 (Linking)
URI: ://000231102400069 http://www.pnas.org/content/102/31/11118.full.pdf
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Title: Proceedings of the National Academy of Sciences of the United States of America
Source Genre: Journal
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Pages: - Volume / Issue: 102 (31) Sequence Number: - Start / End Page: 11118 - 11123 Identifier: -