English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Consequences of the expression of a bacterial glucokinase in potato tubers, both in combination with and independently of a yeast-derived invertase

Fernie, A. R., Riesmeire, J. W., Martiny, A., Ramalingam, S., Willmitzer, L., & Trethewey, R. N. (2000). Consequences of the expression of a bacterial glucokinase in potato tubers, both in combination with and independently of a yeast-derived invertase. In Australian Journal of Plant Physiology (pp. 827-833).

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Fernie, A. R.1, Author              
Riesmeire, J. W.2, Author
Martiny, A.2, Author
Ramalingam, S.2, Author
Willmitzer, L.1, Author              
Trethewey, R. N.3, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              

Content

show
hide
Free keywords: glucokinase hexokinase invertase potato tuber size starch developing cotyledons higher-plants sugar sensor hexokinase glucose growth gene overexpression accumulation purification
 Abstract: The aim of this work was to further define the metabolic factors that regulate carbohydrate metabolism in potato (Solanum tuberosum L. cv. Desiree) tubers. We previously found that glycolysis is induced (and starch accumulation reduced) in transgenic tubers in which a yeast invertase and a glucokinase from Zymomonas mobilis were expressed in the cytosol, whereas potato tuber size is dramatically increased when invertase expression is targeted to the apoplast. In this study we describe the further characterisation of potato tubers expressing a yeast invertase in the apoplast. We also report the generation of two novel transgenic plants in which the Z. mobilis glucokinase gene is expressed tuber-specifically (either in the wild type or apoplastic invertase-expressing background). We evaluated the influence that increasing the glucokinase activity, independent of invertase activity, had on the shift in carbon partitioning, and assessed if the hexoses produced by the apoplastic cleavage of sucrose could be brought into metabolism. We found that expression of glucokinase either in the wild type or in the apoplastic invertase-expressing background led to changes in the levels of glucose and glucose 6-phosphate. However, these changes had little effect on carbon partitioning or tuber size with respect to the parent line. We conclude that neither the accumulation nor the phosphorylation of glucose play a pivotal role in the regulation of metabolism or morphology in the potato tuber.

Details

show
hide
Language(s): eng - English
 Dates: 2000
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000089052400013
URI: ://000089052400013
 Degree: -

Event

show
hide
Title: Australian Journal of Plant Physiology
Place of Event: -
Start-/End Date: -

Legal Case

show

Project information

show

Source 1

show
hide
Title: Australian Journal of Plant Physiology
Source Genre: Proceedings
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 827 - 833 Identifier: -