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  Identification and characterization of plant transporters using heterologous expression systems

Dreyer, I., Horeau, C., Lemaillet, G., Zimmermann, S., Bush, D. R., Rodriguez-Navarro, A., et al. (1999). Identification and characterization of plant transporters using heterologous expression systems. In 11th International Workshop on Plant Membrane Biology (pp. 1073-1087).

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-3096-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-3097-D
Genre: Conference Paper

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 Creators:
Dreyer, I.1, Author              
Horeau, C.2, Author
Lemaillet, G.2, Author
Zimmermann, S.1, Author              
Bush, D. R.2, Author
Rodriguez-Navarro, A.2, Author
Schachtman, D. P.2, Author
Spalding, E. P.2, Author
Sentenac, H.2, Author
Gaber, R. F.2, Author
Affiliations:
1Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753311              
2External Organizations, ou_persistent22              

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Free keywords: membrane transporters transport processes heterologous expression systems transport proteins cloning strategies rectifying potassium channel membrane h+-atpase amino-acid transporters affinity k+ transporter plasma-membrane arabidopsis-thaliana functional expression xenopus-oocytes saccharomyces-cerevisiae molecular-cloning
 Abstract: In recent years major progress has been achieved in the understanding of transport processes in higher plants. The boom in the field of molecular plant physiology led to the identification and characterization of membrane transporters with transport activities for potassium, calcium, sugars, nitrate, ammonium, sulphate, phosphate, amino acids, peptides, and metal ions. Such progress was hardly feasible without heterologous expression of the isolated transport proteins. This review summarizes the different approaches in characterizing plant membrane transporters using heterologous expression systems. By presenting concrete examples, it outlines different cloning strategies, displays the methods used for (i) expression of transport proteins and detection of their function, (ii) biochemical analyses, (iii) explorations of the structure-function relationship through mutational analysis, and concludes with a discussion about the physiological relevance of the analyses in heterologous expression systems.

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Language(s): eng - English
 Dates: 1999
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000081307300016
DOI: 10.1093/jexbot/50.suppl_1.1073
URI: ://000081307300016
 Degree: -

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Title: 11th International Workshop on Plant Membrane Biology
Place of Event: UNIV CAMBRIDGE, CAMBRIDGE, ENGLAND
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Title: 11th International Workshop on Plant Membrane Biology
Source Genre: Proceedings
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Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1073 - 1087 Identifier: -