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  Gas exchange and ultrastructural analysis of transgenic potato plants expressing mRNA antisense construct targeted to the cp-fructose-1,6-bisphosphate phosphatase

Muschak, M., Hoffmann-Benning, S., Fuss, H., Kossmann, J., Willmitzer, L., & Fisahn, J. (1997). Gas exchange and ultrastructural analysis of transgenic potato plants expressing mRNA antisense construct targeted to the cp-fructose-1,6-bisphosphate phosphatase. In 9th Workshop on Photosynthesis (pp. 455-465).

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 Creators:
Muschak, M.1, Author           
Hoffmann-Benning, S.2, Author           
Fuss, H.3, Author
Kossmann, J.4, Author           
Willmitzer, L.2, Author           
Fisahn, J.1, Author           
Affiliations:
1Biophysical Analysis, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753307              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3External Organizations, ou_persistent22              
4Carbohydrate Molecular Physiology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753344              

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Free keywords: calvin-cycle chloroplast fructose-1,6-bisphosphate respiration solanum tuberosum transgenic plants thylakoid ribulose-1,5-bisphosphate carboxylase-oxygenase thaliana l heynh chlorophyll fluorescence alocasia-macrorrhiza arabidopsis-thaliana photosynthetic acclimation impairs photosynthesis co2 assimilation tobacco plants aba mutant
 Abstract: Measurements of the net photosynthetic (P-N), respiration (R) and transpiration (E) rates, leaf conductances (g(H20)) and intercellular CO2 concentrations (C-i), along with the transmission electron microscopy were utilized in order to study differences in photosynthetic traits of transgenic and wild-type (Wt) potato. plants. We used transgenic plants expressing different levels of the chloroplastic isoform of fructose-1,6-bisphosphate phosphatase (FBPase), which had been created via the mRNA antisense technique. A significant reduction of the P-N was found only with FBPase activities below 36 % compared to the Wt, and under irradiances that exceeded 300 mu mol m(-2) s(-1). Progressive attenuation of the FBPase activity caused further P-N reduction. The CO2 compensation concentration and compensation irradiance were identical in the transgenic and Wt plants. Under anoxic conditions a 30 % increase of the P-N occurred in all the plants. The R during the dark period was similar both in the Wt and FBPase-antisense plants. The size and number of starch grains were significantly lower in plants with the FBPase activities below 36 %. Shift to a higher amount of small grana stacks occurred within chloroplasts of the transgenic plants.

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Language(s): eng - English
 Dates: 1997
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:A1997YA11100013
URI: ://A1997YA11100013
 Degree: -

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Title: 9th Workshop on Photosynthesis
Place of Event: WUSTERHAUSEN, GERMANY
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Title: 9th Workshop on Photosynthesis
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 455 - 465 Identifier: -