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  Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis.

Giavalisco, P., Nordhoff, E., Lehrach, H., Gobom, J., & Klose, J. (2003). Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis. In 16th International Symposium on Microscale Separation and Analysis (pp. 207-216).

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-3453-3 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-3454-1
Genre: Conference Paper

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Giavalisco-2003-Extraction of protei.pdf (Any fulltext), 911KB
 
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 Creators:
Giavalisco, P.1, Author           
Nordhoff, E.1, Author
Lehrach, H.1, Author
Gobom, J.1, Author
Klose, J.1, Author
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1External Organizations, ou_persistent22              

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Free keywords: Arabidopsis Proteins/*isolation & purification Chemical Fractionation Chemical Precipitation Chromatin/chemistry Detergents Electrophoresis, Gel, Two-Dimensional/*methods Isoelectric Focusing Silver Staining and Labeling
 Abstract: To increase the number of proteins detectable by two-dimensional electrophoresis (2-DE) in plants, we present a new procedure for extracting total proteins from plant tissue. This method avoids any loss of proteins in the course of sample preparation and results in two different fractions, one comprising mainly the cytoplasmatic proteins, the other one containing predominantly structure bond proteins. 2-DE patterns obtained from these two fractions show that the total number of different protein spots detected exceeds the degree of resolution commonly reported for plant proteins threefold.

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Language(s): eng - English
 Dates: 2003
 Publication Status: Issued
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 Identifiers: ISI: ISI:000180637300028
DOI: 10.1002/elps.200390016
URI: ://000180637300028 http://onlinelibrary.wiley.com/store/10.1002/elps.200390016/asset/207_ftp.pdf?v=1&t=h9qodb8h&s=cfa0836a69f3de1dfcd40f545d2394aa89d9a509
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Title: 16th International Symposium on Microscale Separation and Analysis
Place of Event: SAN DIEGO, CALIFORNIA
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Title: 16th International Symposium on Microscale Separation and Analysis
Source Genre: Proceedings
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 207 - 216 Identifier: -