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Abstract:
Transgenic tobacco plants that express a yeast derived acid invertase in their vacuole were used to analyse the effects of a modification in carbon allocation on cell wall properties and turgor pressure. Significant increases in turgor pressure, free and total acid content were detected in the transgenic lines. Cell wall neutral sugars were examined by gas chromatography and as a result the arabinose concentration was found to be significantly increased in leaf 6 of the transgenic plants. In contrast, fucose was reduced. However, the size exclusion limit of the pectin network was unaffected by the genetic manipulation.