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  Live-cell imaging of ubiquitin-proteasome system function

Hipp, M. S., Bersuker, K., & Kopito, R. R. (2012). Live-cell imaging of ubiquitin-proteasome system function. Methods Mol Biol, 832, 463-72. doi:10.1007/978-1-61779-474-2_33.

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 Creators:
Hipp, M. S.1, Author              
Bersuker, K.2, Author
Kopito, R. R.2, Author
Affiliations:
1Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              
2External Organizations, ou_persistent22              

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Free keywords: Endoplasmic Reticulum/metabolism Flow Cytometry/*methods Fluorescence Genes, Reporter Green Fluorescent Proteins/biosynthesis/genetics HEK293 Cells Humans Proteasome Endopeptidase Complex/*genetics/*metabolism Ubiquitin/chemistry/genetics/metabolism
 Abstract: The role of the ubiquitin-proteasome system (UPS) in maintaining protein homeostasis has generated a demand for assays that quantify UPS function in the presence of chemical and protein UPS inhibitors. Here, we describe protocols that measure changes in UPS reporter levels in response to changes in the expression level, localization, or aggregation state of a second protein. We utilize cell lines stably expressing fluorescent UPS substrates that are transfected with a second protein tagged with a compatible fluorophore. We describe protocols to correlate levels of UPS substrates with changes in the levels or properties of the transfected protein.

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 Dates: 2012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 22350906
DOI: 10.1007/978-1-61779-474-2_33
ISSN: 1940-6029 (Electronic) 1064-3745 (Linking)
URI: http://www.ncbi.nlm.nih.gov/pubmed/22350906
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Title: Methods Mol Biol
Source Genre: Journal
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Pages: - Volume / Issue: 832 Sequence Number: - Start / End Page: 463 - 72 Identifier: -