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  Nanoscopy with more than 100,000 'doughnuts'.

Chmyrov, A., Keller, J., Grotjohann, T., Ratz, M., d'Este, E., Jakobs, S., et al. (2013). Nanoscopy with more than 100,000 'doughnuts'. Nature methods, 10, 737-740. doi:10.1038/nmeth.2556.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1ADC-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-5610-C
Genre: Journal Article

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 Creators:
Chmyrov, A.1, Author              
Keller, J.1, Author              
Grotjohann, T.1, Author              
Ratz, M.2, Author              
d'Este, E.1, Author              
Jakobs, S.2, Author              
Eggeling, C.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_578566              

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 Abstract: We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 μm × 100 μm–sized fields of view in <1 s using 116,000 such doughnuts

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Language(s): eng - English
 Dates: 2013-07-072013-08
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nmeth.2556
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Title: Nature methods
Source Genre: Journal
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Pages: - Volume / Issue: 10 Sequence Number: - Start / End Page: 737 - 740 Identifier: -