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  The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis.

Samwer, M., Dehne, H. J., Spira, F., Kollmar, M., Gerlich, D., Urlaub, H., et al. (2013). The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis. EMBO Journal, 32(13), 1886-1902. doi:10.1038/emboj.2013.108.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1BAB-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-C91E-B
Genre: Journal Article

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 Creators:
Samwer, M.1, Author              
Dehne, H. J., Author
Spira, F., Author
Kollmar, M.2, Author              
Gerlich, D., Author
Urlaub, H.3, Author              
Görlich, D.1, Author              
Affiliations:
1Department of Cellular Logistics, MPI for Biophysical Chemistry, Max Planck Society, ou_578574              
2Research Group of Systems Biology of Motor Proteins, MPI for biophysical chemistry, Max Planck Society, ou_578570              
3Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: cytokinesis; kinesins; meiosis; nuclear actin; phalloidin
 Abstract: Nuclei of Xenopus laevis oocytes grow 100 000-fold larger in volume than a typical somatic nucleus and require an unusual intranuclear F-actin scaffold for mechanical stability. We now developed a method for mapping F-actin interactomes and identified a comprehensive set of F-actin binders from the oocyte nuclei. Unexpectedly, the most prominent interactor was a novel kinesin termed NabKin (Nuclear and meiotic actin-bundling Kinesin). NabKin not only binds microtubules but also F-actin structures, such as the intranuclear actin bundles in prophase and the contractile actomyosin ring during cytokinesis. The interaction between NabKin and F-actin is negatively regulated by Importin-β and is responsive to spatial information provided by RanGTP. Disconnecting NabKin from F-actin during meiosis caused cytokinesis failure and egg polyploidy. We also found actin-bundling activity in Nabkin’s somatic paralogue KIF14, which was previously shown to be essential for somatic cell division. Our data are consistent with the notion that NabKin/KIF14 directly link microtubules with F-actin and that such link is essential for cytokinesis.

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Language(s): eng - English
 Dates: 2013-05-312013-07-03
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/emboj.2013.108
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Title: EMBO Journal
Source Genre: Journal
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Pages: - Volume / Issue: 32 (13) Sequence Number: - Start / End Page: 1886 - 1902 Identifier: -