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Free keywords:
FLUORESCENCE CORRELATION SPECTROSCOPY; EXHIBITING MODEL MEMBRANES;
ATOMIC-FORCE MICROSCOPY; PHOSPHOLIPID MONOLAYERS; PHOSPHATIDYLCHOLINE
MONOLAYERS; UNILAMELLAR VESICLES; PHASE-BEHAVIOR; CHOLESTEROL; BILAYERS;
ELECTROFORMATIONcellular membranes; fluorescence correlation spectroscopy; lipids;
monolayers; protein binding;
Abstract:
Model membrane systems have become invaluable tools to investigate specific features of cellular membranes. Although a variety of different experimental assays does exist, many of them are rather complicated in their preparation and difficult in their practical realisation. Here, we propose a new simple miniaturised monolayer assay that can easily be combined with standard analytical techniques such as confocal fluorescence microscopy and fluorescence correlation spectroscopy (FCS).
