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  NEW TYPE BIOSENSOR FOR THE DETECTION OF PESTICIDES, BASED ON THE INHIBITION OF ACETYLCHOLINESTERASE

Csiffary, G., Nagy, P., Kiss, A., & Adanyi, N. (2013). NEW TYPE BIOSENSOR FOR THE DETECTION OF PESTICIDES, BASED ON THE INHIBITION OF ACETYLCHOLINESTERASE. ACTA ALIMENTARIA, 42(1), 79-90. doi:10.1556/AAlim.42.2013.1.8.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-461F-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-4620-2
Genre: Journal Article

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 Creators:
Csiffary, G.1, Author
Nagy, Peter2, Author              
Kiss, A.1, Author
Adanyi, N.1, Author
Affiliations:
1external, ou_persistent22              
2Hartl, Franz-Ulrich / Cellular Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565152              

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Free keywords: CARBAMATE PESTICIDES; ORGANOPHOSPHATE PESTICIDES; ENZYME-INHIBITION; LIQUID-CHROMATOGRAPHY; GAS-CHROMATOGRAPHY; SAMPLESenzymes; biosensors; amperometric detection; pesticides; thin-layer enzyme cell;
 Abstract: An amperometric biosensor for the detection of organophosphorus and carbamate type pesticides was developed. Acetylcholinesterase enzyme (electric eel) was immobilized in a thin-layer enzyme cell, and acetylthiocholine chloride (AcTCh) was used as substrate. Amperometric detection with glassy carbon electrode was performed in a stopped-flow FIA system. The inhibition effect of dichlorvos (DDVP), carbofuran, methomyl, and pirimicarb were examined: the linear measuring ranges were 0.001-0.1 mu mol l(-1), 0.001-0.1 mu mol l(-1), 0.2-1 mu mol l(-1), and 0.1-10 mu mol l(-1), respectively. Soil extract and apple juice were measured with spiking method. It was concluded that the biosensor can be used for screening pesticide residues in food and environmental samples.

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Language(s): eng - English
 Dates: 2013-03
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Identifiers: ISI: 000321815300008
DOI: 10.1556/AAlim.42.2013.1.8
 Degree: -

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Title: ACTA ALIMENTARIA
Source Genre: Journal
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Publ. Info: PRIELLE K U 19, PO BOX 245,, H-1117 BUDAPEST, HUNGARY : AKADEMIAI KIADO RT
Pages: - Volume / Issue: 42 (1) Sequence Number: - Start / End Page: 79 - 90 Identifier: ISSN: 0139-3006