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  Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops

Freisinger, T., Kluender, B., Johnson, J., Müller, N., Pichler, G., Beck, G., et al. (2013). Establishment of a robust single axis of cell polarity by coupling multiple positive feedback loops. NATURE COMMUNICATIONS, 4: 1807. doi:10.1038/ncomms2795.

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Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-4641-9 Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-4646-0
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 Urheber:
Freisinger, Tina1, Autor              
Kluender, Ben2, Autor
Johnson, Jared2, Autor
Müller, Nikola1, Autor              
Pichler, Garwin1, Autor              
Beck, Gisela1, Autor              
Costanzo, Michael2, Autor
Boone, Charles2, Autor
Cerione, Richard A.2, Autor
Frey, Erwin2, Autor
Wedlich-Söldner, Roland1, Autor              
Affiliations:
1Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              
2external, ou_persistent22              

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Schlagwörter: NUCLEOTIDE EXCHANGE FACTOR; SACCHAROMYCES-CEREVISIAE; SYMMETRY-BREAKING; CDC42 LOCALIZATION; MEMBRANE-PROTEINS; BUDDING YEAST; FACTOR CDC24P; RHO GDI; POLARIZATION; GTPASE
 Zusammenfassung: Establishment of cell polarity-or symmetry breaking-relies on local accumulation of polarity regulators. Although simple positive feedback is sufficient to drive symmetry breaking, it is highly sensitive to stochastic fluctuations typical for living cells. Here, by integrating mathematical modelling with quantitative experimental validations, we show that in the yeast Saccharomyces cerevisiae a combination of actin- and guanine nucleotide dissociation inhibitor-dependent recycling of the central polarity regulator Cdc42 is needed to establish robust cell polarity at a single site during yeast budding. The guanine nucleotide dissociation inhibitor pathway consistently generates a single-polarization site, but requires Cdc42 to cycle rapidly between its active and inactive form, and is therefore sensitive to perturbations of the GTPase cycle. Conversely, actin-mediated recycling of Cdc42 induces robust symmetry breaking but cannot restrict polarization to a single site. Our results demonstrate how cells optimize symmetry breaking through coupling between multiple feedback loops.

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Sprache(n): eng - Englisch
 Datum: 2013-05
 Publikationsstatus: Online publiziert
 Seiten: 11
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: 000320589900003
DOI: 10.1038/ncomms2795
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Titel: NATURE COMMUNICATIONS
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND : NATURE PUBLISHING GROUP
Seiten: - Band / Heft: 4 Artikelnummer: 1807 Start- / Endseite: - Identifikator: ISSN: 2041-1723