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  Interleukin-6 Expression under Gravitational Stress Due to Vibration and Hypergravity in Follicular Thyroid Cancer Cells

Ma, X., Wehland, M., Aleshcheva, G., Hauslage, J., Wasser, K., Hemmersbach, R., et al. (2013). Interleukin-6 Expression under Gravitational Stress Due to Vibration and Hypergravity in Follicular Thyroid Cancer Cells. PLOS ONE, 8(7): e68140. doi:10.1371/journal.pone.0068140.

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 Creators:
Ma, Xiao1, Author
Wehland, Markus1, Author
Aleshcheva, Ganna1, Author
Hauslage, Jens1, Author
Wasser, Kai1, Author
Hemmersbach, Ruth1, Author
Infanger, Manfred1, Author
Bauer, Johann2, Author           
Grimm, Daniela1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: RANDOM POSITIONING MACHINE; PROTEIN-KINASE-C; GENE-EXPRESSION; ENDOTHELIAL-CELLS; GROWTH-FACTOR; SIMULATED MICROGRAVITY; SIGNALING PATHWAYS; PARABOLIC FLIGHT; IL-6 EXPRESSION; CARCINOMA CELLS
 Abstract: It is known that exposing cell lines in vitro to parabolic flights changes their gene expression and protein production patterns. Parabolic flights and spaceflight in general are accompanied by transient hypergravity and vibration, which may impact the cells and therefore, have to be considered too. To estimate the possible impact of transient hypergravity and vibration, we investigated the effects of these forces separately using dedicated ground-based facilities. We placed follicular thyroid ML-1 and CGTH W-1 cancer cells in a specific centrifuge (MuSIC Multi Sample Incubator Centrifuge; SAHC Short Arm Human Centrifuge) simulating the hypergravity phases that occur during one (P1) and 31 parabolas (P31) of parabolic flights, respectively. On the Vibraplex device, the same cell lines were treated with vibration waves corresponding to those that occur during a whole parabolic flight lasting for two hours. After the various treatments, cells were harvested and analyzed by quantitative real-time PCR, focusing on the genes involved in forming (ACTB, MYO9, TUBB, VIM, TLN1, and ITGB1) and modulating (EZR, RDX, and MSN) the cytoskeleton, as well as those encoding growth factors (EGF, CTGF, IL6, and IL8) or protein kinases (PRKAA1 and PRKCA). The analysis revealed alterations in several genes in both cell lines; however, fewer genes were affected in ML-1 than CGTH W-1 cells. Interestingly, IL6 was the only gene whose expression was changed in both cell lines by each treatment, while PKCA transcription remained unaffected in all experiments. We conclude that a PKCa-independent mechanism of IL6 gene activation is very sensitive to physical forces in thyroid cells cultured in vitro as monolayers.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published online
 Pages: 12
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Title: PLOS ONE
Source Genre: Journal
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Publ. Info: 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA : PUBLIC LIBRARY SCIENCE
Pages: - Volume / Issue: 8 (7) Sequence Number: e68140 Start / End Page: - Identifier: ISSN: 1932-6203