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  Systematic identification of proteins binding to chromatin-embedded ubiquitylated H2B reveals recruitment of SWI/SNF to regulate transcription.

Shema-Yaacoby, E., Nikolov, M., Haj-Yahya, M., Siman, P., Allemand, E., Yamaguchi, Y., et al. (2013). Systematic identification of proteins binding to chromatin-embedded ubiquitylated H2B reveals recruitment of SWI/SNF to regulate transcription. Cell Reports, 4(3), 601-608. doi:10.1016/j.celrep.2013.07.014.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-4E3E-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CA7C-2
Genre: Journal Article

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Shema-Yaacoby, E., Author
Nikolov, M.1, Author              
Haj-Yahya, M., Author
Siman, P., Author
Allemand, E., Author
Yamaguchi, Y., Author
Muchardt, C., Author
Urlaub, H.1, Author              
Brik, A., Author
Oren, M., Author
Fischle, W.2, Author              
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              
2Research Group of Chromatin Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578604              

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 Abstract: Chromatin posttranslational modifications (PTMs), including monoubiquitylation of histone H2B on lysine 120 (H2Bub1), play a major role in regulating genome functions. To elucidate the molecular mechanisms of H2Bub1 activity, a chromatin template uniformly containing H2Bub1 was used as an affinity matrix to identify preferentially interacting human proteins. Over 90 such factors were found, including proteins and protein complexes associated with transcription, RNA posttranscriptional modifications, and DNA replication and repair. Notably, we found that the SWI/SNF chromatin remodeling complex associates preferentially with H2Bub1-rich chromatin. Moreover, SWI/SNF is required for optimal transcription of a subset of genes that are selectively dependent on H2Bub1. Our findings substantially expand the known H2Bub1 interactome and provide insights into the functions of this PTM in mammalian gene regulation.

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Language(s): eng - English
 Dates: 2013-08-082013-08-15
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2013.07.014
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Title: Cell Reports
Source Genre: Journal
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Pages: - Volume / Issue: 4 (3) Sequence Number: - Start / End Page: 601 - 608 Identifier: -